論文 - 詳細
| RRC ID | 46149 |
|---|---|
| 著者 | Lee K, Shim J, Bae J, Kim YJ, Lee J. |
| タイトル | Stabilization of RNT-1 protein, runt-related transcription factor (RUNX) protein homolog of Caenorhabditis elegans, by oxidative stress through mitogen-activated protein kinase pathway. |
| ジャーナル | J Biol Chem |
| Abstract |
RUNX proteins are evolutionarily conserved transcription factors known to be involved in various developmental processes. Here we report a new role for a RUNX protein: a role in stress response. We show that RNT-1, the Caenorhabditis elegans RUNX homolog, is constantly produced and degraded by the ubiquitination-proteasome pathway in the intestine of the nematode. RNT-1 was rapidly stabilized by oxidative stress, and the rnt-1-mutant animals were more sensitive to oxidative stress, indicating that rapid RNT-1 stabilization is a defense response against the oxidative stress. The MAP kinase pathway is required for RNT-1 stabilization, and RNT-1 was phosphorylated by SEK-1/PMK-1 in vitro. ChIP-sequencing analysis revealed a feedback loop mechanism of the MAP kinase pathway by the VHP-1 phosphatase in the RNT-1-mediated oxidative stress response. We propose that rnt-1 is regulated at the protein level for its role in the immediate response to environmental challenges in the intestine. |
| 巻・号 | 287(13) |
| ページ | 10444-10452 |
| 公開日 | 2012-3-23 |
| DOI | 10.1074/jbc.M111.314146 |
| PII | S0021-9258(20)65454-0 |
| PMID | 22308034 |
| PMC | PMC3323012 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Core Binding Factor alpha Subunits Dual-Specificity Phosphatases / genetics Dual-Specificity Phosphatases / metabolism Intestinal Mucosa / metabolism* MAP Kinase Signaling System / physiology* Oxidative Stress / physiology* Phosphorylation / physiology Protein Stability Proteolysis* Receptor, EphA4 / genetics Receptor, EphA4 / metabolism Sequence Homology, Amino Acid Transcription Factors / genetics Transcription Factors / metabolism* Ubiquitination / physiology |
| IF | 4.238 |
| 引用数 | 11 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm388 tm491 |