| RRC ID |
4615
|
| Author |
Sakudoh T, Sezutsu H, Nakashima T, Kobayashi I, Fujimoto H, Uchino K, Banno Y, Iwano H, Maekawa H, Tamura T, Kataoka H, Tsuchida K.
|
| Title |
Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Mechanisms for the uptake and transport of carotenoids, essential nutrients for humans, are not well understood in any animal system. The Y (Yellow blood) gene, a critical cocoon color determinant in the silkworm Bombyx mori, controls the uptake of carotenoids into the intestinal mucosa and the silk gland. Here we provide evidence that the Y gene corresponds to the intracellular carotenoid-binding protein (CBP) gene. In the Y recessive strain, the absence of an exon, likely due to an incorrect mRNA splicing caused by a transposon-associated genomic deletion, generates a nonfunctional CBP mRNA, resulting in colorless hemolymph and white cocoons. Enhancement of carotenoid uptake and coloration of the white cocoon was achieved by germ-line transformation with the CBP gene. This study demonstrates the existence of a genetically facilitated intracellular process beyond passive diffusion for carotenoid uptake in the animal phyla, and paves the way for modulating silk color and lipid content through genetic engineering.
|
| Volume |
104(21)
|
| Pages |
8941-6
|
| Published |
2007-5-22
|
| DOI |
10.1073/pnas.0702860104
|
| PII |
0702860104
|
| Description |
Y(黄血）の原因遺伝子はCBPである。CBPはカロテノイド結合タンパク質をコードする。FL50系統(+Y/+Y)では、CBPのエクソン2の3'側169bpが欠失している。
|
| PMID |
17496138
|
| PMC |
PMC1885607
|
| MeSH |
Alleles
Animals
Animals, Genetically Modified
Bombyx / genetics
Bombyx / metabolism
Carotenoids / metabolism*
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Color
Gene Expression Regulation
Genome, Insect / genetics
Insect Proteins / genetics
Insect Proteins / metabolism*
Molecular Sequence Data
Phenotype
Silk / metabolism*
|
| IF |
9.412
|
| Times Cited |
77
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
FL50 |
