論文 - 詳細
| RRC ID | 46201 |
|---|---|
| 著者 | Snoek LB, Sterken MG, Volkers RJ, Klatter M, Bosman KJ, Bevers RP, Riksen JA, Smant G, Cossins AR, Kammenga JE. |
| タイトル | A rapid and massive gene expression shift marking adolescent transition in C. elegans. |
| ジャーナル | Sci Rep |
| Abstract |
Organismal development is the most dynamic period of the life cycle, yet we have only a rough understanding of the dynamics of gene expression during adolescent transition. Here we show that adolescence in Caenorhabditis elegans is characterized by a spectacular expression shift of conserved and highly polymorphic genes. Using a high resolution time series we found that in adolescent worms over 10,000 genes changed their expression. These genes were clustered according to their expression patterns. One cluster involved in chromatin remodelling showed a brief up-regulation around 50 h post-hatch. At the same time a spectacular shift in expression was observed. Sequence comparisons for this cluster across many genotypes revealed diversifying selection. Strongly up-regulated genes showed signs of purifying selection in non-coding regions, indicating that adolescence-active genes are constrained on their regulatory properties. Our findings improve our understanding of adolescent transition and help to eliminate experimental artefacts due to incorrect developmental timing. |
| 巻・号 | 4 |
| ページ | 3912 |
| 公開日 | 2014-1-28 |
| DOI | 10.1038/srep03912 |
| PII | srep03912 |
| PMID | 24468752 |
| PMC | PMC3904150 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Chromosome Mapping Gene Expression Gene Expression Profiling Gene Expression Regulation, Developmental* Genetic Variation Multigene Family / genetics RNA, Messenger / genetics |
| IF | 3.998 |
| 引用数 | 19 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 線虫 | tm1978 |