| RRC ID |
46203
|
| 著者 |
Staab TA, Evgrafov O, Knowles JA, Sieburth D.
|
| タイトル |
Regulation of synaptic nlg-1/neuroligin abundance by the skn-1/Nrf stress response pathway protects against oxidative stress.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
The Nrf family of transcription factors mediates adaptive responses to stress and longevity, but the identities of the crucial Nrf targets, and the tissues in which they function in multicellular organisms to promote survival, are not known. Here, we use whole transcriptome RNA sequencing to identify 810 genes whose expression is controlled by the SKN-1/Nrf2 negative regulator WDR-23 in the nervous system of Caenorhabditis elegans. Among the genes identified is the synaptic cell adhesion molecule nlg-1/neuroligin. We find that the synaptic abundance of NLG-1 protein increases following pharmacological treatments that generate oxidative stress or by the genetic activation of skn-1. Increasing nlg-1 dosage correlates with increased survival in response to oxidative stress, whereas genetic inactivation of nlg-1 reduces survival and impairs skn-1-mediated stress resistance. We identify a canonical SKN-1 binding site in the nlg-1 promoter that binds to SKN-1 in vitro and is necessary for SKN-1 and toxin-mediated increases in nlg-1 expression in vivo. Together, our results suggest that SKN-1 activation in the nervous system can confer protection to organisms in response to stress by directly regulating nlg-1/neuroligin expression.
|
| 巻・号 |
10(1)
|
| ページ |
e1004100
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pgen.1004100
|
| PII |
PGENETICS-D-13-02003
|
| PMID |
24453991
|
| PMC |
PMC3894169
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development
Caenorhabditis elegans Proteins / biosynthesis
Caenorhabditis elegans Proteins / genetics*
Cell Adhesion Molecules, Neuronal / biosynthesis
Cell Adhesion Molecules, Neuronal / genetics*
Cell Survival
DNA-Binding Proteins / biosynthesis
DNA-Binding Proteins / genetics*
Gene Expression Regulation, Developmental
Longevity / genetics*
Nervous System / growth & development
Oxidative Stress / genetics*
Promoter Regions, Genetic
Transcription Factors / biosynthesis
Transcription Factors / genetics*
Transcription, Genetic*
Transcriptional Activation / genetics
|
| IF |
5.175
|
| 引用数 |
25
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 線虫 |
tm1817
tm1108 |
