RRC ID 46214
Author Tserevelakis GJ, Megalou EV, Filippidis G, Petanidou B, Fotakis C, Tavernarakis N.
Title Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.
Journal PLoS ONE
Abstract Elucidation of the molecular mechanisms regulating lipid storage and metabolism is essential for mitigating excess adiposity and obesity, which has been associated with increased prevalence of severe pathological conditions such as cardiovascular disorders and type II diabetes, worldwide. However, imaging fatty acid distribution and dynamics in vivo, at the cellular or organismal level is challenging. We developed a label-free method for visualizing lipid depositions in vivo, based on third harmonic generation (THG) microscopy. THG imaging requires a single pulsed-laser light source, alleviating the technical challenges of implementing coherent anti-Stokes Raman scattering spectroscopy (CARS) to detect fat stores in living cells. We demonstrate that THG can be used to efficiently and reliably visualize lipid droplets in Caenorhabditis elegans. Thus, THG microscopy offers a versatile alternative to fluorescence and dye-based approaches for lipid biology research.
Volume 9(1)
Pages e84431
Published 2014
DOI 10.1371/journal.pone.0084431
PII PONE-D-13-35830
PMID 24392137
PMC PMC3879321
MeSH Animals Caenorhabditis elegans / chemistry* Caenorhabditis elegans / metabolism Lipid Metabolism Lipids / chemistry* Microscopy / methods* Molecular Imaging / methods* Staining and Labeling
IF 2.766
Times Cited 17
C.elegans tm331