RRC ID 46216
著者 Turek M, Lewandrowski I, Bringmann H.
タイトル An AP2 transcription factor is required for a sleep-active neuron to induce sleep-like quiescence in C. elegans.
ジャーナル Curr Biol
Abstract BACKGROUND:Sleep is an essential behavior that is found in all animals that have a nervous system. Neural activity is thought to control sleep, but little is known about the identity and the function of neural circuits underlying sleep. Lethargus is a developmentally regulated period of behavioral quiescence in C. elegans larvae that has sleep-like properties.
RESULTS:We studied sleep-like behavior in C. elegans larvae and found that it requires a highly conserved AP2 transcription factor, aptf-1, which was expressed strongly in only five interneurons in the head. Expression of aptf-1 in one of these neurons, the GABAergic neuron RIS, was required for quiescence. RIS was strongly and acutely activated at the transition from wake-like to sleep-like behavior. Optogenetic activation of aptf-1-expressing neurons ectopically induced acute behavioral quiescence in an aptf-1-dependent manner. RIS ablation caused a dramatic reduction of quiescence. RIS-dependent quiescence, however, does not require GABA but requires neuropeptide signaling.
CONCLUSIONS:We conclude that RIS acts as a sleep-active, sleep-promoting neuron that requires aptf-1 to induce sleep-like behavior through neuropeptide signaling. Sleep-promoting GABAergic-peptidergic neurons have also been identified in vertebrate brains, suggesting that common circuit principles exist between sleep in vertebrates and sleep-like behavior in invertebrates.
巻・号 23(22)
ページ 2215-2223
公開日 2013-11-18
DOI 10.1016/j.cub.2013.09.028
PII S0960-9822(13)01144-5
PMID 24184105
MeSH Animals Behavior, Animal / physiology* Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / physiology* Gene Expression Regulation Interneurons / metabolism Larva Locomotion Mutation Neurons / physiology* Neuropeptides / metabolism Signal Transduction Sleep / physiology Transcription Factor AP-2 / metabolism gamma-Aminobutyric Acid / metabolism
IF 9.601
引用数 54
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm3287