論文 - 詳細
| RRC ID | 46234 |
|---|---|
| 著者 | Wedeles CJ, Wu MZ, Claycomb JM. |
| タイトル | Protection of germline gene expression by the C. elegans Argonaute CSR-1. |
| ジャーナル | Dev Cell |
| Abstract |
In Caenorhabditis elegans, the Piwi-interacting small RNA (piRNA)-mediated germline surveillance system encodes more than 30,000 unique 21-nucleotide piRNAs, which silence a variety of foreign nucleic acids. What mechanisms allow endogenous germline-expressed transcripts to evade silencing by the piRNA pathway? One likely candidate in a protective mechanism is the Argonaute CSR-1, which interacts with 22G-small RNAs that are antisense to nearly all germline-expressed genes. Here, we use an in vivo RNA tethering assay to demonstrate that the recruitment of CSR-1 to a transcript licenses expression of the transcript, protecting it from piRNA-mediated silencing. Licensing occurs mainly at the level of transcription, as we observe changes in pre-mRNA levels consistent with transcriptional activation when CSR-1 is tethered. Furthermore, the recruitment of CSR-1 to a previously silenced locus transcriptionally activates its expression. Together, these results demonstrate a rare positive role for an endogenous Argonaute pathway in heritably licensing and protecting germline transcripts. |
| 巻・号 | 27(6) |
| ページ | 664-71 |
| 公開日 | 2013-12-23 |
| DOI | 10.1016/j.devcel.2013.11.016 |
| PII | S1534-5807(13)00697-7 |
| PMID | 24360783 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromatin Immunoprecipitation Gene Silencing* Germ Cells / metabolism* RNA, Helminth / genetics* RNA, Helminth / metabolism RNA, Messenger / genetics RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Signal Transduction |
| IF | 10.092 |
| 引用数 | 89 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1200 tm892 |