RRC ID 46234
著者 Wedeles CJ, Wu MZ, Claycomb JM.
タイトル Protection of germline gene expression by the C. elegans Argonaute CSR-1.
ジャーナル Dev Cell
Abstract In Caenorhabditis elegans, the Piwi-interacting small RNA (piRNA)-mediated germline surveillance system encodes more than 30,000 unique 21-nucleotide piRNAs, which silence a variety of foreign nucleic acids. What mechanisms allow endogenous germline-expressed transcripts to evade silencing by the piRNA pathway? One likely candidate in a protective mechanism is the Argonaute CSR-1, which interacts with 22G-small RNAs that are antisense to nearly all germline-expressed genes. Here, we use an in vivo RNA tethering assay to demonstrate that the recruitment of CSR-1 to a transcript licenses expression of the transcript, protecting it from piRNA-mediated silencing. Licensing occurs mainly at the level of transcription, as we observe changes in pre-mRNA levels consistent with transcriptional activation when CSR-1 is tethered. Furthermore, the recruitment of CSR-1 to a previously silenced locus transcriptionally activates its expression. Together, these results demonstrate a rare positive role for an endogenous Argonaute pathway in heritably licensing and protecting germline transcripts.
巻・号 27(6)
ページ 664-71
公開日 2013-12-23
DOI 10.1016/j.devcel.2013.11.016
PII S1534-5807(13)00697-7
PMID 24360783
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromatin Immunoprecipitation Gene Silencing* Germ Cells / metabolism* RNA, Helminth / genetics* RNA, Helminth / metabolism RNA, Messenger / genetics RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Signal Transduction
IF 10.092
引用数 89
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1200 tm892