論文 - 詳細
| RRC ID | 46237 |
|---|---|
| 著者 | Williams DC, Bejjani RE, Ramirez PM, Coakley S, Kim SA, Lee H, Wen Q, Samuel A, Lu H, Hilliard MA, Hammarlund M. |
| タイトル | Rapid and permanent neuronal inactivation in vivo via subcellular generation of reactive oxygen with the use of KillerRed. |
| ジャーナル | Cell Rep |
| Abstract |
Inactivation of selected neurons in vivo can define their contribution to specific developmental outcomes, circuit functions, and behaviors. Here, we show that the optogenetic tool KillerRed selectively, rapidly, and permanently inactivates different classes of neurons in C. elegans in response to a single light stimulus, through the generation of reactive oxygen species (ROS). Ablation scales from individual neurons in single animals to multiple neurons in populations and can be applied to freely behaving animals. Using spatially restricted illumination, we demonstrate that localized KillerRed activation in either the cell body or the axon triggers neuronal degeneration and death of the targeted cell. Finally, targeting KillerRed to mitochondria results in organelle fragmentation without killing the cell, in contrast to the cell death observed when KillerRed is targeted to the plasma membrane. We expect this genetic tool to have wide-ranging applications in studies of circuit function and subcellular responses to ROS. |
| 巻・号 | 5(2) |
| ページ | 553-63 |
| 公開日 | 2013-10-31 |
| DOI | 10.1016/j.celrep.2013.09.023 |
| PII | S2211-1247(13)00546-9 |
| PMID | 24209746 |
| PMC | PMC3877846 |
| MeSH | Animals Apoptosis / drug effects Apoptosis / radiation effects Caenorhabditis elegans / metabolism GABAergic Neurons / drug effects GABAergic Neurons / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism* Green Fluorescent Proteins / pharmacology Light Microscopy, Video Reactive Oxygen Species / metabolism* Superoxide Dismutase / metabolism |
| IF | 8.109 |
| 引用数 | 39 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm776 |