RRC ID 46240
著者 Wu YE, Huo L, Maeder CI, Feng W, Shen K.
タイトル The balance between capture and dissociation of presynaptic proteins controls the spatial distribution of synapses.
ジャーナル Neuron
Abstract The location, size, and number of synapses critically influence the specificity and strength of neural connections. In axons, synaptic vesicle (SV) and active zone (AZ) proteins are transported by molecular motors and accumulate at discrete presynaptic loci. Little is known about the mechanisms coordinating presynaptic protein transport and deposition to achieve proper distribution of synaptic material. Here we show that SV and AZ proteins exhibit extensive cotransport and undergo frequent pauses. At the axonal and synaptic pause sites, the balance between the capture and dissociation of mobile transport packets determines the extent of presynaptic assembly. The small G protein ARL-8 inhibits assembly by promoting dissociation, while a JNK kinase pathway and AZ assembly proteins inhibit dissociation. Furthermore, ARL-8 directly binds to the UNC-104/KIF1A motor to limit the capture efficiency. Together, molecular regulation of the dichotomy between axonal trafficking and local assembly controls vital aspects of synapse formation and maintenance.
巻・号 78(6)
ページ 994-1011
公開日 2013-6-19
DOI 10.1016/j.neuron.2013.04.035
PII S0896-6273(13)00366-8
PMID 23727120
PMC PMC3898717
MeSH Animals Animals, Genetically Modified Axons / metabolism Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* GTP Phosphohydrolases / genetics GTP Phosphohydrolases / metabolism* MAP Kinase Signaling System / physiology Presynaptic Terminals / metabolism* Protein Transport / physiology Synapses / genetics Synapses / metabolism*
IF 14.415
引用数 65
WOS 分野 NEUROSCIENCES
リソース情報
線虫 tm2388 tm1125