RRC ID 4625
Author Taketa S, Amano S, Tsujino Y, Sato T, Saisho D, Kakeda K, Nomura M, Suzuki T, Matsumoto T, Sato K, Kanamori H, Kawasaki S, Takeda K.
Title Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a single locus (nud) on chromosome arm 7HL. On the basis of positional cloning, we concluded that an ethylene response factor (ERF) family transcription factor gene controls the covered/naked caryopsis phenotype. This conclusion was validated by (i) fixation of the 17-kb deletion harboring the ERF gene among all 100 naked cultivars studied; (ii) two x-ray-induced nud alleles with a DNA lesion at a different site, each affecting the putative functional motif; and (iii) gene expression strictly localized to the testa. Available results indicate the monophyletic origin of naked barley. The Nud gene has homology to the Arabidopsis WIN1/SHN1 transcription factor gene, whose deduced function is control of a lipid biosynthesis pathway. Staining with a lipophilic dye (Sudan black B) detected a lipid layer on the pericarp epidermis only in covered barley. We infer that, in covered barley, the contact of the caryopsis surface, overlaid with lipids to the inner side of the hull, generates organ adhesion.
Volume 105(10)
Pages 4062-7
Published 2008-3-11
DOI 10.1073/pnas.0711034105
PII 0711034105
PMID 18316719
PMC PMC2268812
MeSH Azo Compounds Cloning, Molecular Edible Grain / metabolism* Gene Expression Regulation, Plant Genes, Plant* Genetic Variation Hordeum / cytology Hordeum / genetics* Hordeum / metabolism* Lipids / analysis Lipids / biosynthesis* Molecular Sequence Data Mutation Naphthalenes Permeability Plant Proteins / genetics* Plant Proteins / metabolism Sequence Analysis, DNA Transcription Factors / genetics* Transcription Factors / metabolism
IF 9.504
Times Cited 112
WOS Category PLANT SCIENCES
Resource
Barley Haruna Nijo(J247) T212 I029 T334 I677 E269 I624 J301 J714 E215 E229 H663 H823 J080 H602 H639 I030 T329 H632 H625 H774 H637 H638 I444 H725 H737