RRC ID |
4625
|
Author |
Taketa S, Amano S, Tsujino Y, Sato T, Saisho D, Kakeda K, Nomura M, Suzuki T, Matsumoto T, Sato K, Kanamori H, Kawasaki S, Takeda K.
|
Title |
Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway.
|
Journal |
Proc Natl Acad Sci U S A
|
Abstract |
In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a single locus (nud) on chromosome arm 7HL. On the basis of positional cloning, we concluded that an ethylene response factor (ERF) family transcription factor gene controls the covered/naked caryopsis phenotype. This conclusion was validated by (i) fixation of the 17-kb deletion harboring the ERF gene among all 100 naked cultivars studied; (ii) two x-ray-induced nud alleles with a DNA lesion at a different site, each affecting the putative functional motif; and (iii) gene expression strictly localized to the testa. Available results indicate the monophyletic origin of naked barley. The Nud gene has homology to the Arabidopsis WIN1/SHN1 transcription factor gene, whose deduced function is control of a lipid biosynthesis pathway. Staining with a lipophilic dye (Sudan black B) detected a lipid layer on the pericarp epidermis only in covered barley. We infer that, in covered barley, the contact of the caryopsis surface, overlaid with lipids to the inner side of the hull, generates organ adhesion.
|
Volume |
105(10)
|
Pages |
4062-7
|
Published |
2008-3-11
|
DOI |
10.1073/pnas.0711034105
|
PII |
0711034105
|
PMID |
18316719
|
PMC |
PMC2268812
|
MeSH |
Azo Compounds
Cloning, Molecular
Edible Grain / metabolism*
Gene Expression Regulation, Plant
Genes, Plant*
Genetic Variation
Hordeum / cytology
Hordeum / genetics*
Hordeum / metabolism*
Lipids / analysis
Lipids / biosynthesis*
Molecular Sequence Data
Mutation
Naphthalenes
Permeability
Plant Proteins / genetics*
Plant Proteins / metabolism
Sequence Analysis, DNA
Transcription Factors / genetics*
Transcription Factors / metabolism
|
IF |
9.412
|
Times Cited |
165
|
WOS Category
|
PLANT SCIENCES
|
Resource |
Barley |
Haruna Nijo(J247)
T212
I029
T334
I677
E269
I624
J301
J714
E215
E229
H663
H823
J080
H602
H639
I030
T329
H632
H625
H774
H637
H638
I444
H725
H737 |