RRC ID 46260
Author Attreed M, Desbois M, van Kuppevelt TH, Bülow HE.
Title Direct visualization of specifically modified extracellular glycans in living animals.
Journal Nat Methods
Abstract Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
Volume 9(5)
Pages 477-9
Published 2012-4-1
DOI 10.1038/nmeth.1945
PII nmeth.1945
PMID 22466794
PMC PMC3437987
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / chemistry* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Green Fluorescent Proteins / chemistry* Green Fluorescent Proteins / genetics Heparitin Sulfate / chemistry* Heparitin Sulfate / genetics Microscopy, Fluorescence / methods Single-Chain Antibodies / chemistry* Single-Chain Antibodies / genetics
IF 30.822
Times Cited 19
C.elegans tm472 tm734 tm3006 tm2051 tm3208