Reference - Detail
RRC ID | 46260 |
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Author | Attreed M, Desbois M, van Kuppevelt TH, Bülow HE. |
Title | Direct visualization of specifically modified extracellular glycans in living animals. |
Journal | Nat Methods |
Abstract |
Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo. |
Volume | 9(5) |
Pages | 477-9 |
Published | 2012-4-1 |
DOI | 10.1038/nmeth.1945 |
PII | nmeth.1945 |
PMID | 22466794 |
PMC | PMC3437987 |
MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / chemistry* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Green Fluorescent Proteins / chemistry* Green Fluorescent Proteins / genetics Heparitin Sulfate / chemistry* Heparitin Sulfate / genetics Microscopy, Fluorescence / methods Single-Chain Antibodies / chemistry* Single-Chain Antibodies / genetics |
IF | 30.822 |
Times Cited | 19 |
WOS Category | BIOCHEMICAL RESEARCH METHODS |
Resource | |
C.elegans | tm472 tm734 tm3006 tm2051 tm3208 |