Reference - Detail
| RRC ID | 46260 |
|---|---|
| Author | Attreed M, Desbois M, van Kuppevelt TH, Bülow HE. |
| Title | Direct visualization of specifically modified extracellular glycans in living animals. |
| Journal | Nat Methods |
| Abstract |
Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo. |
| Volume | 9(5) |
| Pages | 477-9 |
| Published | 2012-4-1 |
| DOI | 10.1038/nmeth.1945 |
| PII | nmeth.1945 |
| PMID | 22466794 |
| PMC | PMC3437987 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / chemistry* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Green Fluorescent Proteins / chemistry* Green Fluorescent Proteins / genetics Heparitin Sulfate / chemistry* Heparitin Sulfate / genetics Microscopy, Fluorescence / methods Single-Chain Antibodies / chemistry* Single-Chain Antibodies / genetics |
| IF | 30.822 |
| Times Cited | 19 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS |
| Resource | |
| C.elegans | tm472 tm734 tm3006 tm2051 tm3208 |