論文 - 詳細
| RRC ID | 46260 |
|---|---|
| 著者 | Attreed M, Desbois M, van Kuppevelt TH, Bülow HE. |
| タイトル | Direct visualization of specifically modified extracellular glycans in living animals. |
| ジャーナル | Nat Methods |
| Abstract |
Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo. |
| 巻・号 | 9(5) |
| ページ | 477-9 |
| 公開日 | 2012-4-1 |
| DOI | 10.1038/nmeth.1945 |
| PII | nmeth.1945 |
| PMID | 22466794 |
| PMC | PMC3437987 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / chemistry* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Green Fluorescent Proteins / chemistry* Green Fluorescent Proteins / genetics Heparitin Sulfate / chemistry* Heparitin Sulfate / genetics Microscopy, Fluorescence / methods Single-Chain Antibodies / chemistry* Single-Chain Antibodies / genetics |
| IF | 30.822 |
| 引用数 | 19 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| リソース情報 | |
| 線虫 | tm472 tm734 tm3006 tm2051 tm3208 |