論文 - 詳細
| RRC ID | 46264 |
|---|---|
| 著者 | Baker RH, Britton C, Roberts B, Loer CM, Matthews JB, Nisbet AJ. |
| タイトル | Melanisation of Teladorsagia circumcincta larvae exposed to sunlight: a role for GTP-cyclohydrolase in nematode survival. |
| ジャーナル | Int J Parasitol |
| Abstract |
Trichostrongylid nematode parasites of livestock inhabit two very different niches during their life-cycle; within the host and free-living in the environment. UV radiation plays a significant role in the survival of free-living, pre-parasitic nematode larvae, with different species exhibiting differing levels of sensitivity. In many eukaryotes, melanisation is a key protective mechanism against UV damage, however there is little information about this process in parasitic nematodes. Caenorhabditis elegans cat-4 mutants, which are deficient in the enzyme guanosine triphosphate-cyclohydrolase I (GTP-CH), have both depleted levels of melanin in their cuticles and an increased sensitivity to anthelmintic drugs. Some parasitic nematodes have very high levels of GTP-CH transcript in their pre-parasitic stages, suggesting an important role for this biopterin synthetic enzyme. Here, we show that the Tci-cat-4 gene, which encodes GTP-CH in Teladorsagia circumcincta, has a role in melanisation and is also capable of rescuing C. elegans cat-4 mutants. In addition, following exposure of T. circumcincta L3s to sunlight, there is a 32% increase in GTP-CH enzyme activity (P=0.019), and a 21% increase in levels of melanin (P=0.031) compared with unexposed larvae. These data suggest that one explanation for the high level of GTP-CH present in pre-parasitic stages of trichostrongylid nematodes is to facilitate melanisation in response to UV exposure. |
| 巻・号 | 42(10) |
| ページ | 887-91 |
| 公開日 | 2012-9-1 |
| DOI | 10.1016/j.ijpara.2012.06.005 |
| PII | S0020-7519(12)00172-5 |
| PMID | 22884628 |
| MeSH | Animals Catalase / genetics Catalase / metabolism GTP Cyclohydrolase / genetics GTP Cyclohydrolase / metabolism* Gene Expression Regulation, Enzymologic / radiation effects* Larva / enzymology Larva / metabolism Larva / radiation effects Melanins / metabolism* Mutation Sunlight* Trichostrongyloidea / enzymology Trichostrongyloidea / metabolism* Trichostrongyloidea / radiation effects* |
| IF | 3.53 |
| 引用数 | 4 |
| WOS 分野 | PARASITOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm773 |