Reference - Detail
| RRC ID | 46281 |
|---|---|
| Author | Castellano-Pozo M, García-Muse T, Aguilera A. |
| Title | R-loops cause replication impairment and genome instability during meiosis. |
| Journal | EMBO Rep |
| Abstract |
R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results. |
| Volume | 13(10) |
| Pages | 923-9 |
| Published | 2012-10-1 |
| DOI | 10.1038/embor.2012.119 |
| PII | embor2012119 |
| PMID | 22878416 |
| PMC | PMC3463965 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA Damage / genetics DNA Replication / genetics* DNA, Fungal / chemistry DNA, Fungal / metabolism Genomic Instability / genetics* Meiosis / genetics* Nuclear Proteins / genetics Nuclear Proteins / metabolism Ribonuclease H / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 7.497 |
| Times Cited | 43 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| C.elegans | tm1310 |