論文 - 詳細
| RRC ID | 46284 |
|---|---|
| 著者 | Cecere G, Zheng GX, Mansisidor AR, Klymko KE, Grishok A. |
| タイトル | Promoters recognized by forkhead proteins exist for individual 21U-RNAs. |
| ジャーナル | Mol Cell |
| Abstract |
C. elegans 21U-RNAs are equivalent to the piRNAs discovered in other metazoans and have important roles in gametogenesis and transposon control. The biogenesis and molecular function of 21U-RNAs and piRNAs are poorly understood. Here, we demonstrate that transcription of each 21U-RNA is regulated separately through a conserved upstream DNA motif. We use genomic analysis to show that this motif is associated with low nucleosome occupancy, a characteristic of many promoters that drive expression of protein-coding genes, and that RNA polymerase II is localized to this nucleosome-depleted region. We establish that the most conserved 8-mer sequence in the upstream region of 21U-RNAs, CTGTTTCA, is absolutely required for their individual expression. Furthermore, we demonstrate that the 8-mer is specifically recognized by Forkhead family (FKH) transcription factors and that 21U-RNA expression is diminished in several FKH mutants. Our results suggest that thousands of small noncoding transcription units are regulated by FKH proteins. |
| 巻・号 | 47(5) |
| ページ | 734-45 |
| 公開日 | 2012-9-14 |
| DOI | 10.1016/j.molcel.2012.06.021 |
| PII | S1097-2765(12)00546-1 |
| PMID | 22819322 |
| PMC | PMC3444671 |
| MeSH | Animals Base Sequence Caenorhabditis elegans / genetics* Forkhead Transcription Factors / metabolism* Gene Expression Profiling Nucleosomes / genetics Promoter Regions, Genetic / genetics* RNA Polymerase II / metabolism RNA, Helminth / genetics* RNA, Small Interfering / genetics* |
| IF | 15.584 |
| 引用数 | 42 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm872 |