| RRC ID |
46284
|
| 著者 |
Cecere G, Zheng GX, Mansisidor AR, Klymko KE, Grishok A.
|
| タイトル |
Promoters recognized by forkhead proteins exist for individual 21U-RNAs.
|
| ジャーナル |
Mol Cell
|
| Abstract |
C. elegans 21U-RNAs are equivalent to the piRNAs discovered in other metazoans and have important roles in gametogenesis and transposon control. The biogenesis and molecular function of 21U-RNAs and piRNAs are poorly understood. Here, we demonstrate that transcription of each 21U-RNA is regulated separately through a conserved upstream DNA motif. We use genomic analysis to show that this motif is associated with low nucleosome occupancy, a characteristic of many promoters that drive expression of protein-coding genes, and that RNA polymerase II is localized to this nucleosome-depleted region. We establish that the most conserved 8-mer sequence in the upstream region of 21U-RNAs, CTGTTTCA, is absolutely required for their individual expression. Furthermore, we demonstrate that the 8-mer is specifically recognized by Forkhead family (FKH) transcription factors and that 21U-RNA expression is diminished in several FKH mutants. Our results suggest that thousands of small noncoding transcription units are regulated by FKH proteins.
|
| 巻・号 |
47(5)
|
| ページ |
734-45
|
| 公開日 |
2012-9-14
|
| DOI |
10.1016/j.molcel.2012.06.021
|
| PII |
S1097-2765(12)00546-1
|
| PMID |
22819322
|
| PMC |
PMC3444671
|
| MeSH |
Animals
Base Sequence
Caenorhabditis elegans / genetics*
Forkhead Transcription Factors / metabolism*
Gene Expression Profiling
Nucleosomes / genetics
Promoter Regions, Genetic / genetics*
RNA Polymerase II / metabolism
RNA, Helminth / genetics*
RNA, Small Interfering / genetics*
|
| IF |
15.584
|
| 引用数 |
42
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm872 |
