RRC ID 46284
著者 Cecere G, Zheng GX, Mansisidor AR, Klymko KE, Grishok A.
タイトル Promoters recognized by forkhead proteins exist for individual 21U-RNAs.
ジャーナル Mol Cell
Abstract C. elegans 21U-RNAs are equivalent to the piRNAs discovered in other metazoans and have important roles in gametogenesis and transposon control. The biogenesis and molecular function of 21U-RNAs and piRNAs are poorly understood. Here, we demonstrate that transcription of each 21U-RNA is regulated separately through a conserved upstream DNA motif. We use genomic analysis to show that this motif is associated with low nucleosome occupancy, a characteristic of many promoters that drive expression of protein-coding genes, and that RNA polymerase II is localized to this nucleosome-depleted region. We establish that the most conserved 8-mer sequence in the upstream region of 21U-RNAs, CTGTTTCA, is absolutely required for their individual expression. Furthermore, we demonstrate that the 8-mer is specifically recognized by Forkhead family (FKH) transcription factors and that 21U-RNA expression is diminished in several FKH mutants. Our results suggest that thousands of small noncoding transcription units are regulated by FKH proteins.
巻・号 47(5)
ページ 734-45
公開日 2012-9-14
DOI 10.1016/j.molcel.2012.06.021
PII S1097-2765(12)00546-1
PMID 22819322
PMC PMC3444671
MeSH Animals Base Sequence Caenorhabditis elegans / genetics* Forkhead Transcription Factors / metabolism* Gene Expression Profiling Nucleosomes / genetics Promoter Regions, Genetic / genetics* RNA Polymerase II / metabolism RNA, Helminth / genetics* RNA, Small Interfering / genetics*
IF 15.584
引用数 42
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm872