RRC ID 46320
Author Raharjo WH, Ghai V, Dineen A, Bastiani M, Gaudet J.
Title Cell architecture: surrounding muscle cells shape gland cell morphology in the Caenorhabditis elegans pharynx.
Journal Genetics
Abstract The acquisition and maintenance of shape is critical for the normal function of most cells. Here we investigate the morphology of the pharyngeal glands of Caenorhabditis elegans. These unicellular glands have long cellular processes that extend discrete lengths through the pharyngeal musculature and terminate at ducts connected to the pharyngeal lumen. From a genetic screen we identified several mutants that affect pharyngeal gland morphology. The most severe such mutant is an allele of sma-1, which encodes a β-spectrin required for embryonic elongation, including elongation of the pharynx. In sma-1 mutants, gland projections form normally but become increasingly abnormal over time, acquiring additional branches, outgrowths, and swelling, suggestive of hypertrophy. Rather than acting in pharyngeal glands, sma-1 functions in the surrounding musculature, suggesting that pharyngeal muscles play a critical role in maintenance of gland morphology by restricting their growth, and analysis of other mutants known to affect pharyngeal muscles supports this hypothesis. We suggest that gland morphology is maintained by a balance of forces from the muscles and the glands.
Volume 189(3)
Pages 885-97
Published 2011-11-1
DOI 10.1534/genetics.111.132449
PII genetics.111.132449
PMID 21868609
PMC PMC3213386
MeSH Alleles Animals Basic Helix-Loop-Helix Transcription Factors / genetics Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / radiation effects Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / genetics DNA Mutational Analysis Gene Expression Regulation / radiation effects Hypertrophy / genetics Lasers / adverse effects Membrane Proteins / genetics Models, Biological Muscle Cells / cytology* Muscle Cells / metabolism Muscle Cells / radiation effects Muscle Cells / ultrastructure Pharyngeal Muscles / cytology* Pharyngeal Muscles / metabolism Pharyngeal Muscles / pathology Pharyngeal Muscles / radiation effects
IF 4.015
Times Cited 7
C.elegans tm299