RRC ID 46378
Author Jose AM, Kim YA, Leal-Ekman S, Hunter CP.
Title Conserved tyrosine kinase promotes the import of silencing RNA into Caenorhabditis elegans cells.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract RNA silencing in Caenorhabditis elegans is transmitted between cells by the transport of double-stranded RNA (dsRNA). The efficiency of such transmission, however, depends on both the cell type and the environment. Here, we identify systemic RNAi defective-3 (SID-3) as a conserved tyrosine kinase required for the efficient import of dsRNA. Without SID-3, cells perform RNA silencing well but import dsRNA poorly. Upon overexpression of SID-3, cells import dsRNA more efficiently than do wild-type cells and such efficient import of dsRNA requires an intact SID-3 kinase domain. The mammalian homolog of SID-3, activated cdc-42-associated kinase (ACK), acts in many signaling pathways that respond to environmental changes and is known to directly associate with endocytic vesicles, which have been implicated in dsRNA transport. Therefore, our results suggest that the SID-3/ACK tyrosine kinase acts as a regulator of RNA import into animal cells.
Volume 109(36)
Pages 14520-5
Published 2012-9-4
DOI 10.1073/pnas.1201153109
PII 1201153109
PMID 22912399
PMC PMC3437824
MeSH Animals Animals, Genetically Modified Base Sequence Biological Transport / physiology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Computational Biology Crosses, Genetic DNA Primers / genetics Genotype Green Fluorescent Proteins Molecular Sequence Data Polymorphism, Single Nucleotide / genetics Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism* RNA Interference / physiology* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Sequence Analysis, DNA
IF 9.504
Times Cited 22
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
C.elegans tm342