Reference - Detail
|Author||Jose AM, Kim YA, Leal-Ekman S, Hunter CP.|
|Title||Conserved tyrosine kinase promotes the import of silencing RNA into Caenorhabditis elegans cells.|
|Journal||Proc. Natl. Acad. Sci. U.S.A.|
RNA silencing in Caenorhabditis elegans is transmitted between cells by the transport of double-stranded RNA (dsRNA). The efficiency of such transmission, however, depends on both the cell type and the environment. Here, we identify systemic RNAi defective-3 (SID-3) as a conserved tyrosine kinase required for the efficient import of dsRNA. Without SID-3, cells perform RNA silencing well but import dsRNA poorly. Upon overexpression of SID-3, cells import dsRNA more efficiently than do wild-type cells and such efficient import of dsRNA requires an intact SID-3 kinase domain. The mammalian homolog of SID-3, activated cdc-42-associated kinase (ACK), acts in many signaling pathways that respond to environmental changes and is known to directly associate with endocytic vesicles, which have been implicated in dsRNA transport. Therefore, our results suggest that the SID-3/ACK tyrosine kinase acts as a regulator of RNA import into animal cells.
|MeSH||Animals Animals, Genetically Modified Base Sequence Biological Transport / physiology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Computational Biology Crosses, Genetic DNA Primers / genetics Genotype Green Fluorescent Proteins Molecular Sequence Data Polymorphism, Single Nucleotide / genetics Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism* RNA Interference / physiology* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Sequence Analysis, DNA|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY|