RRC ID 46407
著者 Meelkop E, Temmerman L, Janssen T, Suetens N, Beets I, Van Rompay L, Shanmugam N, Husson SJ, Schoofs L.
タイトル PDF receptor signaling in Caenorhabditis elegans modulates locomotion and egg-laying.
ジャーナル Mol Cell Endocrinol
Abstract In Caenorhabditis elegans, pdfr-1 encodes three receptors of the secretin receptor family. These G protein-coupled receptors are activated by three neuropeptides, pigment dispersing factors 1a, 1b and 2, which are encoded by pdf-1 and pdf-2. We isolated a PDF receptor loss-of-function allele (lst34) by means of a mutagenesis screen and show that the PDF signaling system is involved in locomotion and egg-laying. We demonstrate that the pdfr-1 mutant phenocopies the defective locomotor behavior of the pdf-1 mutant and that pdf-1 and pdf-2 behave antagonistically. All three PDF receptor splice variants are involved in the regulation of locomotor behavior. Cell specific rescue experiments show that this pdf mediated behavior is regulated by neurons rather than body wall muscles. We also show that egg-laying patterns of pdf-1 and pdf-2 mutants are affected, but not those of pdfr-1 mutants, pointing to a novel role for the PDF-system in the regulation of egg-laying.
巻・号 361(1-2)
ページ 232-40
公開日 2012-9-25
DOI 10.1016/j.mce.2012.05.001
PII S0303-7207(12)00266-3
PMID 22579613
MeSH Animals Behavior, Animal / physiology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* Gene Knockout Techniques Locomotion / physiology* Mutation / genetics Oviposition / physiology* Receptors, G-Protein-Coupled / metabolism* Signal Transduction* Swimming / physiology
IF 3.871
引用数 22
WOS 分野 ENDOCRINOLOGY & METABOLISM CELL BIOLOGY
リソース情報
線虫 tm1996 tm4393 tm4457 tm4780