RRC ID 46424
著者 Phillips CM, Montgomery TA, Breen PC, Ruvkun G.
タイトル MUT-16 promotes formation of perinuclear mutator foci required for RNA silencing in the C. elegans germline.
ジャーナル Genes Dev
Abstract RNA silencing can be initiated by endogenous or exogenously delivered siRNAs. In Caenorhabditis elegans, RNA silencing guided by primary siRNAs is inefficient and therefore requires an siRNA amplification step involving RNA-dependent RNA polymerases (RdRPs). Many factors involved in RNA silencing localize to protein- and RNA-rich nuclear pore-associated P granules in the germline, where they are thought to surveil mRNAs as they exit the nucleus. Mutator class genes are required for siRNA-mediated RNA silencing in both germline and somatic cells, but their specific roles and relationship to other siRNA factors are unclear. Here we show that each of the six mutator proteins localizes to punctate foci at the periphery of germline nuclei. The Mutator foci are adjacent to P granules but are not dependent on core P-granule components or other RNAi pathway factors for their formation or stability. The glutamine/asparagine (Q/N)-rich protein MUT-16 is specifically required for the formation of a protein complex containing the mutator proteins, and in its absence, Mutator foci fail to form at the nuclear periphery. The RdRP RRF-1 colocalizes with MUT-16 at Mutator foci, suggesting a role for Mutator foci in siRNA amplification. Furthermore, we demonstrate that genes that yield high levels of siRNAs, indicative of multiple rounds of siRNA amplification, are disproportionally affected in mut-16 mutants compared with genes that yield low levels of siRNAs. We propose that the mutator proteins and RRF-1 constitute an RNA processing compartment required for siRNA amplification and RNA silencing.
巻・号 26(13)
ページ 1433-44
公開日 2012-7-1
DOI 10.1101/gad.193904.112
PII gad.193904.112
PMID 22713602
PMC PMC3403012
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / genetics Gene Amplification Germ-Line Mutation* RNA Interference*
IF 9.527
引用数 55
WOS 分野 GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1358