論文 - 詳細
| RRC ID | 46439 |
|---|---|
| 著者 | Schvarzstein M, Pattabiraman D, Bembenek JN, Villeneuve AM. |
| タイトル | Meiotic HORMA domain proteins prevent untimely centriole disengagement during Caenorhabditis elegans spermatocyte meiosis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
In many species where oocytes lack centrosomes, sperm contribute both genetic material and centriole(s) to the zygote. Correct centriole organization during male meiosis is critical to guarantee a normal bipolar mitotic spindle in the zygote. During Caenorhabditis elegans male meiosis, centrioles normally undergo two rounds of duplication, resulting in haploid sperm each containing a single tightly engaged centriole pair. Here we identify an unanticipated role for C. elegans HORMA (Hop1/Rev7/Mad2) domain proteins HTP-1/2 and HIM-3 in regulating centriole disengagement during spermatocyte meiosis. In him-3 and htp-1 htp-2 mutants, centrioles separate inappropriately during meiosis II, resulting in spermatids with disengaged centrioles. Moreover, extra centrosomes are detected in a subset of zygotes. Together, these data implicate HIM-3 and HTP-1/2 in preventing centriole disengagement during meiosis II. We showed previously that HTP-1/2 prevents premature loss of sister chromatid cohesion during the meiotic divisions by inhibiting removal of meiotic cohesin complexes containing the REC-8 subunit. Worms lacking REC-8, or expressing a mutant separase protein with elevated local concentration at centrosomes and in sperm, likewise exhibit inappropriate centriole separation during spermatocyte meiosis. These observations are consistent with HIM-3 and HTP-1/2 preventing centriole disengagement by inhibiting separase-dependent cohesin removal. Our data suggest that the same specialized meiotic mechanisms that function to prevent premature release of sister chromatid cohesion during meiosis I in C. elegans also function to inhibit centriole separation at meiosis II, thereby ensuring that the zygote inherits the appropriate complement of chromosomes and centrioles. |
| 巻・号 | 110(10) |
| ページ | E898-907 |
| 公開日 | 2013-3-5 |
| DOI | 10.1073/pnas.1213888110 |
| PII | 1213888110 |
| PMID | 23401519 |
| PMC | PMC3593872 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / physiology Centrioles / physiology Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / physiology Cohesins Female Genes, Helminth Male Meiosis / genetics Meiosis / physiology Models, Biological Mutation Separase Spermatocytes / cytology* Spermatocytes / physiology* Spermatogenesis / genetics Spermatogenesis / physiology |
| IF | 9.412 |
| 引用数 | 15 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2543 |