RRC ID |
46457
|
著者 |
Tzur YB, Egydio de Carvalho C, Nadarajan S, Van Bostelen I, Gu Y, Chu DS, Cheeseman IM, Colaiácovo MP.
|
タイトル |
LAB-1 targets PP1 and restricts Aurora B kinase upon entrance into meiosis to promote sister chromatid cohesion.
|
ジャーナル |
PLoS Biol
|
Abstract |
Successful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific cohesin REC-8 at metaphase I in C. elegans, yet the mechanism and scope of LAB-1 function remained unknown. Here we identify an unexpected earlier role for LAB-1 in promoting the establishment of sister chromatid cohesion in prophase I. LAB-1 and REC-8 are both required for the chromosomal association of the cohesin complex subunit SMC-3. Depletion of lab-1 results in partial loss of sister chromatid cohesion in rec-8 and coh-4 coh-3 mutants and further enhanced chromatid dissociation in worms where all three kleisins are mutated. Moreover, lab-1 depletion results in increased Aurora B kinase (AIR-2) signals in early prophase I nuclei, coupled with a parallel decrease in signals for the PP1 homolog, GSP-2. Finally, LAB-1 directly interacts with GSP-1 and GSP-2. We propose that LAB-1 targets the PP1 homologs to the chromatin at the onset of meiosis I, thereby antagonizing AIR-2 and cooperating with the cohesin complex to promote sister chromatid association and normal progression of the meiotic program.
|
巻・号 |
10(8)
|
ページ |
e1001378
|
公開日 |
2012-1-1
|
DOI |
10.1371/journal.pbio.1001378
|
PII |
PBIOLOGY-D-12-00039
|
PMID |
22927794
|
PMC |
PMC3424243
|
MeSH |
Animals
Aurora Kinase B
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism
Chromatids / genetics
Chromatids / metabolism*
Chromatin / genetics
Chromatin / metabolism
Chromatin Assembly and Disassembly*
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism*
Crossing Over, Genetic
DNA Repair
Meiotic Prophase I*
Protein Binding
Protein Interaction Mapping / methods
Protein Phosphatase 1 / genetics
Protein Phosphatase 1 / metabolism
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism*
RNA Interference
Synaptonemal Complex / genetics
Synaptonemal Complex / metabolism
Two-Hybrid System Techniques
|
IF |
7.076
|
引用数 |
21
|
WOS 分野
|
BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
線虫 |
tm1857
tm3655
tm1791 |