論文 - 詳細
| RRC ID | 46457 |
|---|---|
| 著者 | Tzur YB, Egydio de Carvalho C, Nadarajan S, Van Bostelen I, Gu Y, Chu DS, Cheeseman IM, Colaiácovo MP. |
| タイトル | LAB-1 targets PP1 and restricts Aurora B kinase upon entrance into meiosis to promote sister chromatid cohesion. |
| ジャーナル | PLoS Biol |
| Abstract |
Successful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific cohesin REC-8 at metaphase I in C. elegans, yet the mechanism and scope of LAB-1 function remained unknown. Here we identify an unexpected earlier role for LAB-1 in promoting the establishment of sister chromatid cohesion in prophase I. LAB-1 and REC-8 are both required for the chromosomal association of the cohesin complex subunit SMC-3. Depletion of lab-1 results in partial loss of sister chromatid cohesion in rec-8 and coh-4 coh-3 mutants and further enhanced chromatid dissociation in worms where all three kleisins are mutated. Moreover, lab-1 depletion results in increased Aurora B kinase (AIR-2) signals in early prophase I nuclei, coupled with a parallel decrease in signals for the PP1 homolog, GSP-2. Finally, LAB-1 directly interacts with GSP-1 and GSP-2. We propose that LAB-1 targets the PP1 homologs to the chromatin at the onset of meiosis I, thereby antagonizing AIR-2 and cooperating with the cohesin complex to promote sister chromatid association and normal progression of the meiotic program. |
| 巻・号 | 10(8) |
| ページ | e1001378 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pbio.1001378 |
| PII | PBIOLOGY-D-12-00039 |
| PMID | 22927794 |
| PMC | PMC3424243 |
| MeSH | Animals Aurora Kinase B Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Chromatids / genetics Chromatids / metabolism* Chromatin / genetics Chromatin / metabolism Chromatin Assembly and Disassembly* Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Crossing Over, Genetic DNA Repair Meiotic Prophase I* Protein Binding Protein Interaction Mapping / methods Protein Phosphatase 1 / genetics Protein Phosphatase 1 / metabolism Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* RNA Interference Synaptonemal Complex / genetics Synaptonemal Complex / metabolism Two-Hybrid System Techniques |
| IF | 7.076 |
| 引用数 | 21 |
| WOS 分野 | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1857 tm3655 tm1791 |