RRC ID 46478
Author Zanetti S, Grinschgl S, Meola M, Belfiore M, Rey S, Bianchi P, Puoti A.
Title The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA.
Journal RNA
Abstract Post-transcriptional control regulates many aspects of germline development in the Caenorhabditis elegans hermaphrodite. This nematode switches from spermatogenesis to oogenesis and is, therefore, capable of self-fertilization. This sperm-oocyte switch requires 3' UTR-mediated repression of the fem-3 mRNA. Loss of fem-3 repression results in continuous spermatogenesis in hermaphrodites. Although several factors regulating fem-3 have been identified, little is known about the mechanisms that control fem-3. Here, we investigate the steady-state levels of the fem-3 transcript and the expression pattern of its protein product. We show that FEM-3 is exclusively present in germ cells that are committed to spermatogenesis. We found that in fem-3(gf)/+ heterozygotes, mutant fem-3 gain-of-function transcripts are more abundant than their wild-type counterpart. Furthermore, we show that the penetrance of the fem-3(gf) allele correlates with inefficient FBF binding and extended poly(A) tail size of fem-3 mRNAs. Finally, we show that wild-type and gain-of-function mutated fem-3 mRNAs associate equally well with polyribosomes. We propose that the fem-3 mRNA is regulated through stabilization rather than through translatability.
Volume 18(7)
Pages 1385-94
Published 2012-7-1
DOI 10.1261/rna.031237.111
PII rna.031237.111
PMID 22635404
PMC PMC3383969
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Female Gene Expression Gene Expression Regulation, Developmental Genes, Switch* Hermaphroditic Organisms / genetics* Heterozygote Male Oocytes / growth & development* Oogenesis / genetics Oogenesis / physiology Protein Binding RNA, Messenger / metabolism* RNA-Binding Proteins / metabolism* Spermatogenesis / genetics Spermatogenesis / physiology Spermatozoa / growth & development*
IF 4.32
Times Cited 7
C.elegans tm2435 tm1312