論文 - 詳細
| RRC ID | 46478 |
|---|---|
| 著者 | Zanetti S, Grinschgl S, Meola M, Belfiore M, Rey S, Bianchi P, Puoti A. |
| タイトル | The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA. |
| ジャーナル | RNA |
| Abstract |
Post-transcriptional control regulates many aspects of germline development in the Caenorhabditis elegans hermaphrodite. This nematode switches from spermatogenesis to oogenesis and is, therefore, capable of self-fertilization. This sperm-oocyte switch requires 3' UTR-mediated repression of the fem-3 mRNA. Loss of fem-3 repression results in continuous spermatogenesis in hermaphrodites. Although several factors regulating fem-3 have been identified, little is known about the mechanisms that control fem-3. Here, we investigate the steady-state levels of the fem-3 transcript and the expression pattern of its protein product. We show that FEM-3 is exclusively present in germ cells that are committed to spermatogenesis. We found that in fem-3(gf)/+ heterozygotes, mutant fem-3 gain-of-function transcripts are more abundant than their wild-type counterpart. Furthermore, we show that the penetrance of the fem-3(gf) allele correlates with inefficient FBF binding and extended poly(A) tail size of fem-3 mRNAs. Finally, we show that wild-type and gain-of-function mutated fem-3 mRNAs associate equally well with polyribosomes. We propose that the fem-3 mRNA is regulated through stabilization rather than through translatability. |
| 巻・号 | 18(7) |
| ページ | 1385-94 |
| 公開日 | 2012-7-1 |
| DOI | 10.1261/rna.031237.111 |
| PII | rna.031237.111 |
| PMID | 22635404 |
| PMC | PMC3383969 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Female Gene Expression Gene Expression Regulation, Developmental Genes, Switch* Hermaphroditic Organisms / genetics* Heterozygote Male Oocytes / growth & development* Oogenesis / genetics Oogenesis / physiology Protein Binding RNA, Messenger / metabolism* RNA-Binding Proteins / metabolism* Spermatogenesis / genetics Spermatogenesis / physiology Spermatozoa / growth & development* |
| IF | 4.32 |
| 引用数 | 7 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2435 tm1312 |