論文 - 詳細
| RRC ID | 46493 |
|---|---|
| 著者 | Zhang Y, Wang H, Kage-Nakadai E, Mitani S, Wang X. |
| タイトル | C. elegans secreted lipid-binding protein NRF-5 mediates PS appearance on phagocytes for cell corpse engulfment. |
| ジャーナル | Curr Biol |
| Abstract |
BACKGROUND:During programmed cell death, apoptotic cells are rapidly removed by phagocytes. How dying cells are recognized remains poorly understood. RESULTS:Here we identify a secreted lipid transfer/LPS-binding family protein, NRF-5, which is required for efficient clearance of cell corpses. We observed that phosphatidylserine (PS), which is externalized to the outer leaflet of plasma membranes in apoptotic cells, is also detected on the surface of engulfing cells. Loss of NRF-5 function completely blocks PS appearance on engulfing cells but causes accumulation of PS on apoptotic cells, a phenotype observed in both ced-7(lf) and ttr-52(lf) mutants. The NRF-5 protein is expressed in and secreted from body wall muscle cells and clusters around apoptotic cells in a CED-7-dependent manner. NRF-5 associates with TTR-52, binds PS, and displays lipid transfer activity in vitro. CONCLUSION:Our data suggest that NRF-5 may act with CED-7 and TTR-52 to mediate PS transfer from apoptotic cells to engulfing cells and thus promotes engulfment by phagocytes. |
| 巻・号 | 22(14) |
| ページ | 1276-84 |
| 公開日 | 2012-7-24 |
| DOI | 10.1016/j.cub.2012.06.004 |
| PII | S0960-9822(12)00644-6 |
| PMID | 22727700 |
| MeSH | Animals Apoptosis* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Embryo, Nonmammalian / metabolism Phagocytes / metabolism Phosphatidylserines / metabolism* Sequence Alignment Sequence Analysis, DNA |
| IF | 9.601 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2078 |