RRC ID 46516
著者 Temmerman L, Bogaerts A, Meelkop E, Cardoen D, Boerjan B, Janssen T, Schoofs L.
タイトル A proteomic approach to neuropeptide function elucidation.
ジャーナル Peptides
Abstract Many of the diverse functions of neuropeptides are still elusive. As they are ideally suited to modulate traditional signaling, their added actions are not always detectable under standard laboratory conditions. The search for function assignment to peptide encoding genes can therefore greatly benefit from molecular information. Specific molecular changes resulting from neuropeptide signaling may direct researchers to yet unknown processes or conditions, for which studying these signaling systems may eventually lead to phenotypic confirmation. Here, we applied gel-based proteomics after pdf-1 neuropeptide gene knockout in the model organism Caenorhabditis elegans. It has previously been described that pdf-1 null mutants display a locomotion defect, being slower and making more turns and reversals than wild type worms. The vertebrate functional homolog of PDF-1, vasocative intestinal peptide (VIP), is known to influence a plethora of processes, which have so far not been investigated for pdf-1. Because proteins represent the actual effectors inside an organism, proteomic analysis can guide our view to novel pdf-1 actions in the nematode worm. Our data show that knocking out pdf-1 results in alteration of levels of proteins involved in fat metabolism, stress resistance and development. This indicates a possible conservation of VIP-like actions for pdf-1 in C. elegans.
巻・号 34(1)
ページ 3-9
公開日 2012-3-1
DOI 10.1016/j.peptides.2011.08.025
PII S0196-9781(11)00344-5
PMID 21920396
MeSH Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Neurobiology / methods Neuropeptides / metabolism* Proteomics / methods*
IF 2.843
引用数 6
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY ENDOCRINOLOGY & METABOLISM PHARMACOLOGY & PHARMACY
リソース情報
線虫 tm1996