RRC ID 46521
著者 Topf U, Chiquet-Ehrismann R.
タイトル Genetic interaction between Caenorhabditis elegans teneurin ten-1 and prolyl 4-hydroxylase phy-1 and their function in collagen IV-mediated basement membrane integrity during late elongation of the embryo.
ジャーナル Mol Biol Cell
Abstract Teneurins are a family of phylogenetically conserved proteins implicated in pattern formation and morphogenesis. The sole orthologue in Caenorhabditis elegans, ten-1, is important for hypodermal cell migration, neuronal migration, path finding and fasciculation, gonad development, and basement membrane integrity of some tissues. However, the mechanisms of TEN-1 action remain to be elucidated. Using a genome-wide RNA interference approach, we identified phy-1 as a novel interaction partner of ten-1. phy-1 codes for the catalytic domain of collagen prolyl 4-hydroxylase. Loss of phy-1 significantly enhanced the embryonic lethality of ten-1 null mutants. Double-mutant embryos arrested during late elongation with epidermal defects, disruption of basement membranes, and detachment of body wall muscles. We found that deletion of phy-1 caused aggregation of collagen IV in body wall muscles in elongated embryos and triggered the loss of tissue integrity in ten-1 mutants. In addition, phy-1 and ten-1 each genetically interact with genes encoding collagen IV. These findings support a functional mechanism in which loss of ten-1, together with a reduction of assembled and secreted basement membrane collagen IV protein, leads to detachment of the epidermis from muscle cells during late elongation of the embryo when mechanical stress is generated by muscle contractions.
巻・号 22(18)
ページ 3331-43
公開日 2011-9-1
DOI 10.1091/mbc.E10-10-0853
PII mbc.E10-10-0853
PMID 21795395
PMC PMC3172259
MeSH Animals Basement Membrane / abnormalities Basement Membrane / metabolism* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Collagen Type IV / metabolism* Epidermis / abnormalities Epidermis / metabolism Epistasis, Genetic* Gene Knockdown Techniques Genes, Lethal Larva / genetics Larva / growth & development Larva / metabolism Membrane Proteins / genetics* Membrane Proteins / metabolism Muscles / abnormalities Muscles / metabolism Procollagen-Proline Dioxygenase / genetics* Procollagen-Proline Dioxygenase / metabolism RNA Interference
IF 3.791
引用数 14
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm651