論文 - 詳細
| RRC ID | 46526 |
|---|---|
| 著者 | Wagner J, Allman E, Taylor A, Ulmschneider K, Kovanda T, Ulmschneider B, Nehrke K, Peters MA. |
| タイトル | A calcineurin homologous protein is required for sodium-proton exchange events in the C. elegans intestine. |
| ジャーナル | Am J Physiol Cell Physiol |
| Abstract |
Caenorhabditis elegans defecation is a rhythmic behavior, composed of three sequential muscle contractions, with a 50-s periodicity. The motor program is driven by oscillatory calcium signaling in the intestine. Proton fluxes, which require sodium-proton exchangers at the apical and basolateral intestinal membranes, parallel the intestinal calcium flux. These proton shifts are critical for defecation-associated muscle contraction, nutrient uptake, and longevity. How sodium-proton exchangers are activated in time with intestinal calcium oscillation is not known. The posterior body defecation contraction mutant (pbo-1) encodes a calcium-binding protein with homology to calcineurin homologous proteins, which are putative cofactors for mammalian sodium-proton exchangers. Loss of pbo-1 function results in a weakened defecation muscle contraction and a caloric restriction phenotype. Both of these phenotypes also arise from dysfunctions in pH regulation due to mutations in intestinal sodium-proton exchangers. Dynamic, in vivo imaging of intestinal proton flux in pbo-1 mutants using genetically encoded pH biosensors demonstrates that proton movements associated with these sodium-proton exchangers are significantly reduced. The basolateral acidification that signals the first defecation motor contraction is scant in the mutant compared with a normal animal. Luminal and cytoplasmic pH shifts are much reduced in the absence of PBO-1 compared with control animals. We conclude that pbo-1 is required for normal sodium-proton exchanger activity and may couple calcium and proton signaling events. |
| 巻・号 | 301(6) |
| ページ | C1389-403 |
| 公開日 | 2011-12-1 |
| DOI | 10.1152/ajpcell.00139.2011 |
| PII | ajpcell.00139.2011 |
| PMID | 21865588 |
| PMC | PMC3273995 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Calcineurin / genetics Calcineurin / metabolism* Calcium / metabolism Defecation / physiology* Intestines / physiology* Muscle Contraction / physiology* Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Sodium-Hydrogen Exchangers / genetics Sodium-Hydrogen Exchangers / metabolism* |
| IF | 3.485 |
| 引用数 | 11 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm3716 |