| RRC ID |
46526
|
| 著者 |
Wagner J, Allman E, Taylor A, Ulmschneider K, Kovanda T, Ulmschneider B, Nehrke K, Peters MA.
|
| タイトル |
A calcineurin homologous protein is required for sodium-proton exchange events in the C. elegans intestine.
|
| ジャーナル |
Am J Physiol Cell Physiol
|
| Abstract |
Caenorhabditis elegans defecation is a rhythmic behavior, composed of three sequential muscle contractions, with a 50-s periodicity. The motor program is driven by oscillatory calcium signaling in the intestine. Proton fluxes, which require sodium-proton exchangers at the apical and basolateral intestinal membranes, parallel the intestinal calcium flux. These proton shifts are critical for defecation-associated muscle contraction, nutrient uptake, and longevity. How sodium-proton exchangers are activated in time with intestinal calcium oscillation is not known. The posterior body defecation contraction mutant (pbo-1) encodes a calcium-binding protein with homology to calcineurin homologous proteins, which are putative cofactors for mammalian sodium-proton exchangers. Loss of pbo-1 function results in a weakened defecation muscle contraction and a caloric restriction phenotype. Both of these phenotypes also arise from dysfunctions in pH regulation due to mutations in intestinal sodium-proton exchangers. Dynamic, in vivo imaging of intestinal proton flux in pbo-1 mutants using genetically encoded pH biosensors demonstrates that proton movements associated with these sodium-proton exchangers are significantly reduced. The basolateral acidification that signals the first defecation motor contraction is scant in the mutant compared with a normal animal. Luminal and cytoplasmic pH shifts are much reduced in the absence of PBO-1 compared with control animals. We conclude that pbo-1 is required for normal sodium-proton exchanger activity and may couple calcium and proton signaling events.
|
| 巻・号 |
301(6)
|
| ページ |
C1389-403
|
| 公開日 |
2011-12-1
|
| DOI |
10.1152/ajpcell.00139.2011
|
| PII |
ajpcell.00139.2011
|
| PMID |
21865588
|
| PMC |
PMC3273995
|
| MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Calcineurin / genetics
Calcineurin / metabolism*
Calcium / metabolism
Defecation / physiology*
Intestines / physiology*
Muscle Contraction / physiology*
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Sodium-Hydrogen Exchangers / genetics
Sodium-Hydrogen Exchangers / metabolism*
|
| IF |
3.485
|
| 引用数 |
11
|
|
WOS 分野
|
PHYSIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm3716 |
