RRC ID 46533
Author Wynne DJ, Rog O, Carlton PM, Dernburg AF.
Title Dynein-dependent processive chromosome motions promote homologous pairing in C. elegans meiosis.
Journal J Cell Biol
Abstract Meiotic chromosome segregation requires homologue pairing, synapsis, and crossover recombination, which occur during meiotic prophase. Telomere-led chromosome motion has been observed or inferred to occur during this stage in diverse species, but its mechanism and function remain enigmatic. In Caenorhabditis elegans, special chromosome regions known as pairing centers (PCs), rather than telomeres, associate with the nuclear envelope (NE) and the microtubule cytoskeleton. In this paper, we investigate chromosome dynamics in living animals through high-resolution four-dimensional fluorescence imaging and quantitative motion analysis. We find that chromosome movement is constrained before meiosis. Upon prophase onset, constraints are relaxed, and PCs initiate saltatory, processive, dynein-dependent motions along the NE. These dramatic motions are dispensable for homologous pairing and continue until synapsis is completed. These observations are consistent with the idea that motions facilitate pairing by enhancing the search rate but that their primary function is to trigger synapsis. This quantitative analysis of chromosome dynamics in a living animal extends our understanding of the mechanisms governing faithful genome inheritance.
Volume 196(1)
Pages 47-64
Published 2012-1-9
DOI 10.1083/jcb.201106022
PII jcb.201106022
PMID 22232701
PMC PMC3255982
MeSH Actins / physiology Animals Biological Transport Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology Chromosome Segregation* Chromosomes / physiology Chromosomes / ultrastructure Dyneins / metabolism Dyneins / physiology* Meiosis / genetics* Microscopy, Fluorescence
IF 8.811
Times Cited 58
C.elegans tm611