RRC ID 46561
著者 Aguirre-Chen C, Bülow HE, Kaprielian Z.
タイトル C. elegans bicd-1, homolog of the Drosophila dynein accessory factor Bicaudal D, regulates the branching of PVD sensory neuron dendrites.
ジャーナル Development
Abstract The establishment of cell type-specific dendritic arborization patterns is a key phase in the assembly of neuronal circuitry that facilitates the integration and processing of synaptic and sensory input. Although studies in Drosophila and vertebrate systems have identified a variety of factors that regulate dendrite branch formation, the molecular mechanisms that control this process remain poorly defined. Here, we introduce the use of the Caenorhabditis elegans PVD neurons, a pair of putative nociceptors that elaborate complex dendritic arbors, as a tractable model for conducting high-throughput RNAi screens aimed at identifying key regulators of dendritic branch formation. By carrying out two separate RNAi screens, a small-scale candidate-based screen and a large-scale screen of the ~3000 genes on chromosome IV, we retrieved 11 genes that either promote or suppress the formation of PVD-associated dendrites. We present a detailed functional characterization of one of the genes, bicd-1, which encodes a microtubule-associated protein previously shown to modulate the transport of mRNAs and organelles in a variety of organisms. Specifically, we describe a novel role for bicd-1 in regulating dendrite branch formation and show that bicd-1 is likely to be expressed, and primarily required, in PVD neurons to control dendritic branching. We also present evidence that bicd-1 operates in a conserved pathway with dhc-1 and unc-116, components of the dynein minus-end-directed and kinesin-1 plus-end-directed microtubule-based motor complexes, respectively, and interacts genetically with the repulsive guidance receptor unc-5.
巻・号 138(3)
ページ 507-18
公開日 2011-2-1
DOI 10.1242/dev.060939
PII 138/3/507
PMID 21205795
PMC PMC3014636
MeSH Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cytoplasmic Dyneins / genetics Cytoplasmic Dyneins / metabolism Dendrites / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Kinesins / genetics Kinesins / metabolism Protein Binding RNA Interference Sensory Receptor Cells / cytology* Sensory Receptor Cells / metabolism*
IF 5.611
引用数 34
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm3421