Reference - Detail
| RRC ID | 46568 |
|---|---|
| Author | Baudrimont A, Penkner A, Woglar A, Machacek T, Wegrostek C, Gloggnitzer J, Fridkin A, Klein F, Gruenbaum Y, Pasierbek P, Jantsch V. |
| Title | Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans. |
| Journal | PLoS Genet |
| Abstract |
The Caenorhabditis elegans inner nuclear envelope protein matefin/SUN-1 plays a conserved, pivotal role in the process of genome haploidization. CHK-2-dependent phosphorylation of SUN-1 regulates homologous chromosome pairing and interhomolog recombination in Caenorhabditis elegans. Using time-lapse microscopy, we characterized the movement of matefin/SUN-1::GFP aggregates (the equivalent of chromosomal attachment plaques) and showed that the dynamics of matefin/SUN-1 aggregates remained unchanged throughout leptonene/zygotene, despite the progression of pairing. Movement of SUN-1 aggregates correlated with chromatin polarization. We also analyzed the requirements for the formation of movement-competent matefin/SUN-1 aggregates in the context of chromosome structure and found that chromosome axes were required to produce wild-type numbers of attachment plaques. Abrogation of synapsis led to a deceleration of SUN-1 aggregate movement. Analysis of matefin/SUN-1 in a double-strand break deficient mutant revealed that repair intermediates influenced matefin/SUN-1 aggregate dynamics. Investigation of movement in meiotic regulator mutants substantiated that proper orchestration of the meiotic program and effective repair of DNA double-strand breaks were necessary for the wild-type behavior of matefin/SUN-1 aggregates. |
| Volume | 6(11) |
| Pages | e1001219 |
| Published | 2010-11-24 |
| DOI | 10.1371/journal.pgen.1001219 |
| PMID | 21124819 |
| PMC | PMC2991264 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / metabolism* Cell Nucleus / metabolism Chromatin / metabolism Chromosomes / metabolism* Cytoskeleton / metabolism DNA Breaks, Double-Stranded Genotype Meiotic Prophase I* Mitosis Models, Biological Nuclear Proteins / metabolism* Protein Structure, Quaternary Protein Transport Receptors, Cytoplasmic and Nuclear / chemistry Receptors, Cytoplasmic and Nuclear / metabolism* Synaptonemal Complex / metabolism |
| IF | 5.175 |
| Times Cited | 47 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| C.elegans | tm2144 |