論文 - 詳細
| RRC ID | 46574 |
|---|---|
| 著者 | Calixto A, Chelur D, Topalidou I, Chen X, Chalfie M. |
| タイトル | Enhanced neuronal RNAi in C. elegans using SID-1. |
| ジャーナル | Nat Methods |
| Abstract |
We expressed SID-1, a transmembrane protein from Caenorhabditis elegans that is required for systemic RNA interference (RNAi), in C. elegans neurons. This expression increased the response of neurons to double-stranded (ds)RNA delivered by feeding. Mutations in the lin-15b and lin-35 genes enhanced this effect. Worms expressing neuronal SID-1 showed RNAi phenotypes when fed with bacteria expressing dsRNA for known neuronal genes and for uncharacterized genes with no previously known neuronal phenotypes. Neuronal expression of sid-1 decreased nonneuronal RNAi, suggesting that neurons expressing transgenic sid-1(+) served as a sink for dsRNA. This effect, or a sid-1(-) background, can be used to uncover neuronal defects for lethal genes. Expression of sid-1(+) from cell-specific promoters in sid-1 mutants results in cell-specific feeding RNAi. We used these strains to identify a role for integrin signaling genes in mechanosensation. |
| 巻・号 | 7(7) |
| ページ | 554-9 |
| 公開日 | 2010-7-1 |
| DOI | 10.1038/nmeth.1463 |
| PII | nmeth.1463 |
| PMID | 20512143 |
| PMC | PMC2894993 |
| MeSH | Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation / physiology Genes, Lethal Membrane Proteins / genetics Membrane Proteins / metabolism* Neurons / metabolism* RNA Interference / physiology* Repressor Proteins / genetics Repressor Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 30.822 |
| 引用数 | 181 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2109 |