論文 - 詳細
| RRC ID | 46575 |
|---|---|
| 著者 | Chatzigeorgiou M, Grundy L, Kindt KS, Lee WH, Driscoll M, Schafer WR. |
| タイトル | Spatial asymmetry in the mechanosensory phenotypes of the C. elegans DEG/ENaC gene mec-10. |
| ジャーナル | J Neurophysiol |
| Abstract |
DEG/ENaC channels have been broadly implicated in mechanosensory transduction, yet many questions remain about how these proteins contribute to complexes that sense mechanical stimuli. In C. elegans, two DEG/ENaC channel subunits are thought to contribute to a gentle touch transduction complex: MEC-4, which is essential for gentle touch sensation, and MEC-10, whose importance is less well defined. By characterizing a mec-10 deletion mutant, we have found that MEC-10 is important, but not essential, for gentle touch responses in the body touch neurons ALM, PLM, and PVM. Surprisingly, the requirement for MEC-10 in ALM and PLM is spatially asymmetric; mec-10 animals show significant behavioral and physiological responses to stimulation at the distal end of touch neuron dendrites, but respond poorly to stimuli applied near the neuronal cell body. The subcellular distribution of a rescuing MEC-10::GFP translational fusion was found to be restricted to the neuronal cell body and proximal dendrite, consistent with the hypothesis that MEC-10 protein is asymmetrically distributed within the touch neuron process. These results suggest that MEC-10 may contribute to only a subset of gentle touch mechanosensory complexes found preferentially at the proximal dendrite. |
| 巻・号 | 104(6) |
| ページ | 3334-44 |
| 公開日 | 2010-12-1 |
| DOI | 10.1152/jn.00330.2010 |
| PII | jn.00330.2010 |
| PMID | 20881202 |
| PMC | PMC3007656 |
| MeSH | Alleles Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Calcium / analysis Dendrites / chemistry Gene Deletion Green Fluorescent Proteins / analysis Mechanotransduction, Cellular / physiology* Membrane Proteins / deficiency Membrane Proteins / genetics Membrane Proteins / physiology* Recombinant Fusion Proteins / analysis Sensory Receptor Cells / physiology* Sensory Receptor Cells / ultrastructure Subcellular Fractions / chemistry Touch / physiology* |
| IF | 2.234 |
| 引用数 | 15 |
| WOS 分野 | PHYSIOLOGY NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1552 |