RRC ID 46579
Author Conine CC, Batista PJ, Gu W, Claycomb JM, Chaves DA, Shirayama M, Mello CC.
Title Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans.
Journal Proc Natl Acad Sci U S A
Abstract Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at elevated temperature. Here we describe the role of two AGO-class paralogs, alg-3 (T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP::alg-3 transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis. alg-3/4 double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs. These findings add to a growing number of AGO pathways required for thermotolerant fertility in C. elegans and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks.
Volume 107(8)
Pages 3588-93
Published 2010-2-23
DOI 10.1073/pnas.0911685107
PII 0911685107
PMID 20133686
PMC PMC2840486
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Fertility / genetics Fertility / physiology* Hot Temperature Male Mutation RNA, Small Interfering / biosynthesis* RNA, Small Interfering / genetics RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Spermatogenesis / genetics Spermatogenesis / physiology* Spermatozoa / metabolism*
IF 9.412
Times Cited 106
C.elegans tm1155 tm1184 tm1860