論文 - 詳細
| RRC ID | 4660 |
|---|---|
| 著者 | Shuaibu MN, Wuyep PA, Yanagi T, Hirayama K, Tanaka T, Kouno I. |
| タイトル | The use of microfluorometric method for activity-guided isolation of antiplasmodial compound from plant extracts. |
| ジャーナル | Parasitol Res |
| Abstract |
In vitro antiplasmodial activity of methanolic extracts of 16 medicinal plants was evaluated by fluorometric assay using PicoGreen. The IC50s, as determined by parasite DNA concentration, ranged from <11 to >200 and <13 to >200 microg/ml for Plasmodium falciparum 3D7 and K1, respectively; and the most active extracts were those from Anogeissus leiocarpus and Terminalia avicennoides (<11-> or =14 microg/ml). Aqueous, butanolic, ethyl acetate, and methanolic fractions of these two extracts revealed butanolic fraction to have a relatively better activity (IC50, 10-12 microg/ml). Activity-guided chromatographic separation of the butanolic fraction on Sephadex LH-20 followed by nuclear magnetic resonance and correlation high-performance liquid chromatography revealed the presence of known hydrolysable tannins and some related compounds-castalagin, ellagic acid, flavogallonic acid, punicalagin, terchebulin, and two other fractions. The IC50s of all these compounds ranged between 8-21 microg/ml (8-40 microM) against both the strains. Toxicity assay with mouse fibroblasts showed all the extracts and isolated compounds to have IC50 > or = 1500 microg/ml, except for Momordica balsamina with <1500 microg/l. All the extracts and isolated compounds did not affect the integrity of human erythrocyte membrane at the observed IC50s. However, adverse effects manifest in a concentration-dependent fashion (from IC50 > or = 500 microg/ml). |
| 巻・号 | 102(6) |
| ページ | 1119-27 |
| 公開日 | 2008-5-1 |
| DOI | 10.1007/s00436-008-0879-6 |
| PMID | 18214539 |
| MeSH | Animals Antimalarials / chemistry Antimalarials / isolation & purification* Antimalarials / pharmacology* Antimalarials / toxicity Cells, Cultured Chromatography, High Pressure Liquid Erythrocytes / drug effects Fibroblasts / drug effects Fluorometry / methods* Humans Inhibitory Concentration 50 Magnetic Resonance Spectroscopy Mice Molecular Structure Plant Extracts / chemistry* Plants, Medicinal / chemistry Plasmodium falciparum / drug effects* |
| IF | 1.641 |
| 引用数 | 35 |
| WOS 分野 | PARASITOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | Plasmodium falciparum |