RRC ID 4660
Author Shuaibu MN, Wuyep PA, Yanagi T, Hirayama K, Tanaka T, Kouno I.
Title The use of microfluorometric method for activity-guided isolation of antiplasmodial compound from plant extracts.
Journal Parasitol. Res.
Abstract In vitro antiplasmodial activity of methanolic extracts of 16 medicinal plants was evaluated by fluorometric assay using PicoGreen. The IC50s, as determined by parasite DNA concentration, ranged from <11 to >200 and <13 to >200 microg/ml for Plasmodium falciparum 3D7 and K1, respectively; and the most active extracts were those from Anogeissus leiocarpus and Terminalia avicennoides (<11-> or =14 microg/ml). Aqueous, butanolic, ethyl acetate, and methanolic fractions of these two extracts revealed butanolic fraction to have a relatively better activity (IC50, 10-12 microg/ml). Activity-guided chromatographic separation of the butanolic fraction on Sephadex LH-20 followed by nuclear magnetic resonance and correlation high-performance liquid chromatography revealed the presence of known hydrolysable tannins and some related compounds-castalagin, ellagic acid, flavogallonic acid, punicalagin, terchebulin, and two other fractions. The IC50s of all these compounds ranged between 8-21 microg/ml (8-40 microM) against both the strains. Toxicity assay with mouse fibroblasts showed all the extracts and isolated compounds to have IC50 > or = 1500 microg/ml, except for Momordica balsamina with <1500 microg/l. All the extracts and isolated compounds did not affect the integrity of human erythrocyte membrane at the observed IC50s. However, adverse effects manifest in a concentration-dependent fashion (from IC50 > or = 500 microg/ml).
Volume 102(6)
Pages 1119-27
Published 2008-5
DOI 10.1007/s00436-008-0879-6
PMID 18214539
MeSH Animals Antimalarials / chemistry Antimalarials / isolation & purification* Antimalarials / pharmacology* Antimalarials / toxicity Cells, Cultured Chromatography, High Pressure Liquid Erythrocytes / drug effects Fibroblasts / drug effects Fluorometry / methods* Humans Inhibitory Concentration 50 Magnetic Resonance Spectroscopy Mice Molecular Structure Plant Extracts / chemistry* Plants, Medicinal / chemistry Plasmodium falciparum / drug effects*
IF 2.558
Times Cited 22
Pathogenic microorganisms Plasmodium falciparum