論文 - 詳細
| RRC ID | 46666 |
|---|---|
| 著者 | Rapti G, Richmond J, Bessereau JL. |
| タイトル | A single immunoglobulin-domain protein required for clustering acetylcholine receptors in C. elegans. |
| ジャーナル | EMBO J |
| Abstract |
At Caenorhabditis elegans neuromuscular junctions (NMJs), synaptic clustering of the levamisole-sensitive acetylcholine receptors (L-AChRs) relies on an extracellular scaffold assembled in the synaptic cleft. It involves the secreted protein LEV-9 and the ectodomain of the transmembrane protein LEV-10, which are both expressed by muscle cells. L-AChRs, LEV-9 and LEV-10 are part of a physical complex, which localizes at NMJs, yet none of its components localizes independently at synapses. In a screen for mutants partially resistant to the cholinergic agonist levamisole, we identified oig-4, which encodes a small protein containing a single immunoglobulin domain. The OIG-4 protein is secreted by muscle cells and physically interacts with the L-AChR/LEV-9/LEV-10 complex. Removal of OIG-4 destabilizes the complex and causes a loss of L-AChR clusters at the synapse. Interestingly, OIG-4 partially localizes at NMJs independently of LEV-9 and LEV-10, thus providing a potential link between the L-AChR-associated scaffold and local synaptic cues. These results add a novel paradigm for the immunoglobulin super-family as OIG-4 is a secreted protein required for clustering ionotropic receptors independently of synapse formation. |
| 巻・号 | 30(4) |
| ページ | 706-18 |
| 公開日 | 2011-2-16 |
| DOI | 10.1038/emboj.2010.355 |
| PII | emboj2010355 |
| PMID | 21252855 |
| PMC | PMC3041951 |
| MeSH | Animals Animals, Genetically Modified Anthelmintics / pharmacology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cholinergic Agonists / pharmacology Drug Resistance / genetics Immunoglobulins / chemistry Levamisole / pharmacology Models, Biological Neuromuscular Junction / genetics Neuromuscular Junction / metabolism Protein Binding Protein Structure, Tertiary / physiology Protein Transport Receptors, Cholinergic / genetics Receptors, Cholinergic / metabolism* Tissue Distribution |
| IF | 9.889 |
| 引用数 | 25 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm3753 |