論文 - 詳細
RRC ID | 46700 |
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著者 | Zand TP, Reiner DJ, Der CJ. |
タイトル | Ras effector switching promotes divergent cell fates in C. elegans vulval patterning. |
ジャーナル | Dev Cell |
Abstract |
The C. elegans vulva is patterned by epidermal growth factor (EGF) activation of Ras to control 1° fate, and 1° fate induces antagonistic Notch-dependent 2° fate. Furthermore, a spatial EGF gradient, in addition to inducing 1° fate, directly contributes to 2° fate via an unknown pathway. We find that in addition to its canonical effector, Raf, vulval Ras utilizes an exchange factor for the Ral small GTPase (RalGEF), such that Ras-RalGEF-Ral antagonizes Ras-Raf pro-1° fate activity. Consistent with its restricted expression pattern, Ral participates in EGF pro-2° activity. Thus, we have delineated a Ras effector-switching mechanism whereby position within the morphogen gradient dictates that Ras effector usage is switched to RalGEF from Raf to promote 2° instead of 1° fate. Our observations define the utility of Ras effector switching during normal development and may provide a possible mechanistic basis for cell and cancer-type differences in effector dependency and activation. |
巻・号 | 20(1) |
ページ | 84-96 |
公開日 | 2011-1-18 |
DOI | 10.1016/j.devcel.2010.12.004 |
PII | S1534-5807(10)00585-X |
PMID | 21238927 |
PMC | PMC3028984 |
MeSH | Amino Acid Sequence Animals Body Patterning* Caenorhabditis elegans / chemistry Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Cell Lineage* Epidermal Growth Factor / metabolism ErbB Receptors / metabolism Female Models, Biological Molecular Sequence Data Receptors, Notch / metabolism Sequence Alignment Sequence Homology, Amino Acid Signal Transduction Vulva / cytology* Vulva / growth & development* Vulva / metabolism raf Kinases / metabolism ral Guanine Nucleotide Exchange Factor / chemistry ral Guanine Nucleotide Exchange Factor / metabolism ras Proteins / metabolism* |
IF | 10.092 |
引用数 | 32 |
WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
リソース情報 | |
線虫 | tm2760 |