Reference - Detail
| RRC ID | 4671 |
|---|---|
| Author | Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, Hayashi M. |
| Title | Polyubiquitin promoter-based binary vectors for overexpression and gene silencing in Lotus japonicus. |
| Journal | Mol Plant Microbe Interact |
| Abstract |
In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using beta-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus. |
| Volume | 21(4) |
| Pages | 375-82 |
| Published | 2008-4-1 |
| DOI | 10.1094/MPMI-21-4-0375 |
| PMID | 18321183 |
| MeSH | Caulimovirus / genetics Gene Silencing* Genetic Vectors / genetics* Lotus / cytology Lotus / genetics* Models, Genetic Plant Leaves / genetics Plant Roots / genetics Plant Stems / genetics Plants, Genetically Modified Pollen / genetics Polyubiquitin / genetics* Promoter Regions, Genetic / genetics* Root Nodules, Plant / genetics |
| IF | 3.696 |
| Times Cited | 70 |
| WOS Category | PLANT SCIENCES BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 20 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Lotus / Glycine | Miyakojima MG-20 pUB vector |