| RRC ID |
4671
|
| Author |
Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, Hayashi M.
|
| Title |
Polyubiquitin promoter-based binary vectors for overexpression and gene silencing in Lotus japonicus.
|
| Journal |
Mol Plant Microbe Interact
|
| Abstract |
In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using beta-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.
|
| Volume |
21(4)
|
| Pages |
375-82
|
| Published |
2008-4-1
|
| DOI |
10.1094/MPMI-21-4-0375
|
| PMID |
18321183
|
| MeSH |
Caulimovirus / genetics
Gene Silencing*
Genetic Vectors / genetics*
Lotus / cytology
Lotus / genetics*
Models, Genetic
Plant Leaves / genetics
Plant Roots / genetics
Plant Stems / genetics
Plants, Genetically Modified
Pollen / genetics
Polyubiquitin / genetics*
Promoter Regions, Genetic / genetics*
Root Nodules, Plant / genetics
|
| IF |
3.696
|
| Times Cited |
70
|
|
WOS Category
|
PLANT SCIENCES
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Lotus / Glycine |
Miyakojima MG-20
pUB vector |
