RRC ID 46736
Author Park SK, Link CD, Johnson TE.
Title Life-span extension by dietary restriction is mediated by NLP-7 signaling and coelomocyte endocytosis in C. elegans.
Journal FASEB J
Abstract Recent studies have shown that the rate of aging can be modulated by diverse interventions. Dietary restriction is the most widely used intervention to promote longevity; however, the mechanisms underlying the effect of dietary restriction remain elusive. In a previous study, we identified two novel genes, nlp-7 and cup-4, required for normal longevity in Caenorhabditis elegans. nlp-7 is one of a set of neuropeptide-like protein genes; cup-4 encodes an ion-channel involved in endocytosis by coelomocytes. Here, we assess whether nlp-7 and cup-4 mediate longevity increases by dietary restriction. RNAi of nlp-7 or cup-4 significantly reduces the life span of the eat-2 mutant, a genetic model of dietary restriction, but has no effect on the life span of long-lived mutants resulting from reduced insulin/IGF-1 signaling or dysfunction of the mitochondrial electron transport chain. The life-span extension observed in wild-type N2 worms by dietary restriction using bacterial dilution is prevented significantly in nlp-7 and cup-4 mutants. RNAi knockdown of genes encoding candidate receptors of NLP-7 and genes involved in endocytosis by coelomocytes also specifically shorten the life span of the eat-2 mutant. We conclude that two novel pathways, NLP-7 signaling and endocytosis by coelomocytes, are required for life extension under dietary restriction in C. elegans.
Volume 24(2)
Pages 383-92
Published 2010-2-1
DOI 10.1096/fj.09-142984
PII fj.09-142984
PMID 19783783
PMC PMC2812037
MeSH Aging / drug effects Aging / physiology Animals Caenorhabditis elegans / cytology Caenorhabditis elegans Proteins / physiology* Caloric Restriction Endocytosis / physiology* Longevity / genetics* Neuropeptides / physiology* Oxidative Stress RNA Interference Signal Transduction / physiology*
IF 4.966
Times Cited 33
C.elegans tm2984 tm2990