RRC ID 46736
Author Park SK, Link CD, Johnson TE.
Title Life-span extension by dietary restriction is mediated by NLP-7 signaling and coelomocyte endocytosis in C. elegans.
Journal FASEB J.
Abstract Recent studies have shown that the rate of aging can be modulated by diverse interventions. Dietary restriction is the most widely used intervention to promote longevity; however, the mechanisms underlying the effect of dietary restriction remain elusive. In a previous study, we identified two novel genes, nlp-7 and cup-4, required for normal longevity in Caenorhabditis elegans. nlp-7 is one of a set of neuropeptide-like protein genes; cup-4 encodes an ion-channel involved in endocytosis by coelomocytes. Here, we assess whether nlp-7 and cup-4 mediate longevity increases by dietary restriction. RNAi of nlp-7 or cup-4 significantly reduces the life span of the eat-2 mutant, a genetic model of dietary restriction, but has no effect on the life span of long-lived mutants resulting from reduced insulin/IGF-1 signaling or dysfunction of the mitochondrial electron transport chain. The life-span extension observed in wild-type N2 worms by dietary restriction using bacterial dilution is prevented significantly in nlp-7 and cup-4 mutants. RNAi knockdown of genes encoding candidate receptors of NLP-7 and genes involved in endocytosis by coelomocytes also specifically shorten the life span of the eat-2 mutant. We conclude that two novel pathways, NLP-7 signaling and endocytosis by coelomocytes, are required for life extension under dietary restriction in C. elegans.
Volume 24(2)
Pages 383-92
Published 2010-2
DOI 10.1096/fj.09-142984
PII fj.09-142984
PMID 19783783
PMC PMC2812037
MeSH Aging / drug effects Aging / physiology Animals Caenorhabditis elegans / cytology Caenorhabditis elegans Proteins / physiology* Caloric Restriction Endocytosis / physiology* Longevity / genetics* Neuropeptides / physiology* Oxidative Stress RNA Interference Signal Transduction / physiology*
IF 5.595
Times Cited 22
C.elegans tm2984 tm2990