RRC ID 46737
著者 Peters N, Perez DE, Song MH, Liu Y, Müller-Reichert T, Caron C, Kemphues KJ, O'Connell KF.
タイトル Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1.
ジャーナル J Cell Sci
Abstract Centriole duplication is of crucial importance during both mitotic and male meiotic divisions, but it is currently not known whether this process is regulated differently during the two modes of division. In Caenorhabditis elegans, the kinase ZYG-1 plays an essential role in both mitotic and meiotic centriole duplication. We have found that the C-terminus of ZYG-1 is necessary and sufficient for targeting to centrosomes and is important for differentiating mitotic and meiotic centriole duplication. Small truncations of the C-terminus dramatically lower the level of ZYG-1 at mitotic centrosomes but have little effect on the level of ZYG-1 at meiotic centrosomes. Interestingly, truncation of ZYG-1 blocks centrosome duplication in the mitotic cycle but leads to centrosome amplification in the meiotic cycle. Meiotic centriole amplification appears to result from the overduplication of centrioles during meiosis I and leads to the formation of multipolar meiosis II spindles. The extra centrioles also disrupt spermatogenesis by inducing the formation of supernumerary fertilization-competent spermatids that contain abnormal numbers of chromosomes and centrioles. Our data reveal differences in the regulation of mitotic and meiotic centrosome duplication, particularly with regard to ZYG-1 activity, and reveal an important role for centrosomes in spermatid formation.
巻・号 123(Pt 5)
ページ 795-805
公開日 2010-3-1
DOI 10.1242/jcs.050682
PII jcs.050682
PMID 20144993
PMC PMC2823580
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Centrioles / genetics Centrioles / metabolism* Centrosome / metabolism Immunoblotting Male Meiosis / genetics Meiosis / physiology* Microscopy, Confocal Microscopy, Electron Mitosis / genetics Mitosis / physiology* Protein Kinases / genetics Protein Kinases / metabolism* Spermatocytes / cytology Spermatocytes / metabolism
IF 4.573
引用数 18
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1598