RRC ID 46782
Author Suzuki R, Yikelamu A, Tanaka R, Igawa K, Yokozeki H, Yaguchi T.
Title Studies in Phylogeny, Development of Rapid IdentificationMethods, Antifungal Susceptibility, and Growth Rates of Clinical Strains of Sporothrix schenckii Complex in Japan.
Journal Med Mycol J
Abstract Sporotrichosis is a fungal infection caused by the Sporothrix species, which have distinct virulence profiles and geographic distributions. We performed a phylogenetic study in strains morphologically identified as Sporothrix schenckii from clinical specimens in Japan, which were preserved at the Medical Mycology Research Center, Chiba University. In addition, we examined the in vitro antifungal susceptibility and growth rate to evaluate their physiological features. Three hundred strains were examined using sequence analysis of the partial calmodulin gene, or polymerase chain reaction(PCR)method using newly designed species-specific primers; 291 strains were Sporothrix globosa and 9 strains were S. schenckii sensu stricto (in narrow sense, s. s.). S. globosa strains were further clustered into two subclades, and S. schenckii s. s. strains were divided into three subclades. In 38 strains of S. globosa for which antifungal profiles were determined, 4 strains (11%) showed high minimal inhibitory concentration (MIC) value for itraconazole. All tested strains of S. schenckii s. s. and S. globosa showed low sensitivity for amphotericin B. These antifungals are used for treatment of sporotrichosis when infection is severe. S. schenckii s. s. grew better than S. globosa; wherein S. globosa showed restricted growth at 35℃ and did not grow at 37℃. Our molecular data showed that S. globosa is the main causal agent of sporotrichosis in Japan. It is important to determine the antifungal profiles of each case, in addition to accurate species-level identification, to strategize the therapy for sporotrichosis.
Volume 57(3)
Pages E47-57
Published 2016-1-1
DOI 10.3314/mmj.16-00005
PMID 27581775
MeSH Antifungal Agents / pharmacology* Antifungal Agents / therapeutic use Calmodulin / genetics Drug Resistance, Fungal Humans Japan Microbial Sensitivity Tests* Microbiological Techniques / methods* Mycology / methods* Phylogeny* Polymerase Chain Reaction / methods Sequence Analysis Sporothrix / drug effects* Sporothrix / genetics Sporothrix / growth & development Sporothrix / isolation & purification* Sporotrichosis / drug therapy Sporotrichosis / microbiology
Pathogenic microorganisms IFM 5890 IFM 5891 IFM 5897 IFM 5912 IFM 5930 IFM 5949 IFM 5986 IFM 6015 IFM 6046 IFM 46302 IFM 41598 IFM 46307 IFM 46350 IFM 46360 IFM 46361 IFM 50961 IFM 50962 IFM 50963 IFM 50967 IFM 50968 IFM 50969 IFM 50972 IFM 50974 IFM 50975 IFM 50976 IFM 50977 IFM 50979 IFM 50980 IFM 53929 IFM 54251 IFM 55052 IFM 55519 IFM 55520 IFM 55594 IFM 57547 IFM 58046 IFM 58055 IFM 58089 IFM 58181 IFM 58182 IFM 58187 IFM 58191 IFM 58499 IFM 59345 IFM 59466 IFM 60241 IFM 60377 IFM 60781 IFM 60896 IFM 61322 IFM 62244 IFM 62673 IFM 62727 IFM 63176 IFM 63204 IFM 63340