| RRC ID |
467
|
| Author |
Bao Y, Mega K, Yamano Y, Morishima I.
|
| Title |
cDNA cloning and expression of bacteria-induced Hdd11 gene from eri-silkworm, Samia cynthia ricini.
|
| Journal |
Comp Biochem Physiol C Toxicol Pharmacol
|
| Abstract |
A cDNA clone encoding Hdd11 protein, a bacteria-induced protein of unknown function, was isolated from fat body of immunized Samia cynthia ricini larvae based on suppression subtractive hybridization. The cDNA encodes a 167 amino acid residue open reading frame with an 18 residue predicted signal peptide. The deduced amino acid sequence showed 54% and 55% identity with Hdd11 proteins from Hyphantria cunea and Manduca sexta, respectively. Expression of the gene was undetectable in naive larvae when measured by Northern blot hybridization, but strongly induced in fat body after injecting bacterial cells or peptidoglycan into the larvae. The mRNA expression in the fat body was detected as early as 3 h post injection, reached peak levels at 12 h and continued for further 60 h at significant levels. The transcript was detected at very low levels in midgut, hemocytes and malpighian tubules.
|
| Volume |
136(4)
|
| Pages |
337-42
|
| Published |
2003-12-1
|
| DOI |
10.1016/j.cca.2003.10.007
|
| PII |
S1532045603002394
|
| PMID |
15012905
|
| MeSH |
Amino Acid Sequence
Animals
Bacteria
Base Sequence
Bombyx / genetics*
Cloning, Molecular*
DNA, Complementary / genetics*
Fat Body / chemistry
Gene Expression
Genes, Insect*
Insect Proteins / chemistry
Insect Proteins / isolation & purification
Insect Proteins / metabolism*
Molecular Sequence Data
Open Reading Frames
Protein Sorting Signals
RNA, Messenger / metabolism
Sequence Homology, Amino Acid
Time Factors
Tissue Distribution
|
| IF |
2.897
|
| Times Cited |
22
|
|
WOS Category
|
ZOOLOGY
TOXICOLOGY
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
Samia cynthia ricini |
