RRC ID 4680
Author Maekawa-Yoshikawa M, Müller J, Takeda N, Maekawa T, Sato S, Tabata S, Perry J, Wang TL, Groth M, Brachmann A, Parniske M.
Title The temperature-sensitive brush mutant of the legume Lotus japonicus reveals a link between root development and nodule infection by rhizobia.
Journal Plant Physiol.
Abstract The brush mutant of Lotus japonicus exhibits a temperature-dependent impairment in nodule, root, and shoot development. At 26 degrees C, brush formed fewer nodules, most of which were not colonized by rhizobia bacteria. Primary root growth was retarded and the anatomy of the brush root apical meristem revealed distorted cellular organization and reduced cell expansion. Reciprocal grafting of brush with wild-type plants indicated that this genotype only affected the root and that the shoot phenotype was a secondary effect. The root and nodulation phenotype cosegregated as a single Mendelian trait and the BRUSH gene could be mapped to the short arm of chromosome 2. At 18 degrees C, the brush root anatomy was rescued and similar to the wild type, and primary root length, number of infection threads, and nodule formation were partially rescued. Superficially, the brush root phenotype resembled the ethylene-related thick short root syndrome. However, treatment with ethylene inhibitor did not recover the observed phenotypes, although brush primary roots were slightly longer. The defects of brush in root architecture and infection thread development, together with intact nodule architecture and complete absence of symptoms from shoots, suggest that BRUSH affects cellular differentiation in a tissue-dependent way.
Volume 149(4)
Pages 1785-96
Published 2009-4
DOI 10.1104/pp.108.135160
PII pp.108.135160
PMID 19176723
PMC PMC2663734
MeSH Abscisic Acid / pharmacology Chromosomes, Plant / genetics Ethylenes / pharmacology Genes, Plant* Genotype Gibberellins / pharmacology Indoleacetic Acids / pharmacology Lotus / drug effects Lotus / genetics* Lotus / growth & development Lotus / microbiology Mutation / genetics* Phenotype Plant Shoots / cytology Plant Shoots / drug effects Plant Shoots / microbiology Rhizobium / drug effects Rhizobium / physiology* Root Nodules, Plant / cytology Root Nodules, Plant / drug effects Root Nodules, Plant / growth & development* Root Nodules, Plant / microbiology* Sucrose / pharmacology Temperature*
IF 6.305
Times Cited 19
Lotus / Glycine Gifu B-129 Miyakojima MG-20 M. loti STM