RRC ID 46849
Author Ishikawa T, Itoh F, Yoshida S, Saijo S, Matsuzawa T, Gonoi T, Saito T, Okawa Y, Shibata N, Miyamoto T, Yamasaki S.
Title Identification of distinct ligands for the C-type lectin receptors Mincle and Dectin-2 in the pathogenic fungus Malassezia.
Journal Cell Host Microbe
Abstract Various C-type lectin receptors (CLRs), including Mincle and Dectin-2, function as pattern recognition receptors and play a central role in immunity to fungal pathogens. However, the precise structures of the CLR ligands in various pathogenic fungi have yet to be completely defined. Here we report that Malassezia, an opportunistic skin fungal pathogen, is cooperatively recognized by Mincle and Dectin-2 through distinct ligands. Solvent-based fractionation revealed that Mincle and Dectin-2 recognize lipophilic and hydrophilic components of Malassezia, respectively. Mass spectrometry and nuclear magnetic resonance (NMR) revealed glyceroglycolipid and unique mannosyl fatty acids linked to mannitol as two Mincle ligands. An O-linked mannobiose-rich glycoprotein was identified as a Malassezia ligand for Dectin-2. Cytokine production in response to the Mincle ligands and the Dectin-2 ligand was abrogated in Mincle(-/-) and Dectin-2(-/-) dendritic cells, respectively. These results demonstrate that Mincle and Dectin-2 recognize distinct ligands in Malassezia to induce host immune responses.
Volume 13(4)
Pages 477-88
Published 2013-4-17
DOI 10.1016/j.chom.2013.03.008
PII S1931-3128(13)00118-2
PMID 23601109
MeSH Animals Cytokines / immunology Cytokines / metabolism Fungi / immunology* Fungi / metabolism Glycolipids / immunology Glycolipids / metabolism Glycoproteins / immunology Glycoproteins / metabolism Lectins, C-Type / immunology* Lectins, C-Type / metabolism Ligands Malassezia / immunology* Malassezia / metabolism Mannose / immunology Mannose / metabolism Membrane Proteins / immunology* Membrane Proteins / metabolism Mice Mice, Inbred C57BL Receptors, Mitogen / immunology* Receptors, Mitogen / metabolism
IF 15.923
Times Cited 111
Pathogenic microorganisms IFM 48586 IFM 52635