RRC ID 4687
Author Ichida H, Yoneyama K, Koba T, Abe T.
Title Epigenetic modification of rhizobial genome is essential for efficient nodulation.
Journal Biochem. Biophys. Res. Commun.
Abstract CcrM is one of the solitary bacterial DNA methyltransferases which does not have corresponding restriction enzymes. We established a stable ccrM-overexpressing mutant of Mesorhizobium loti, MlccrM-OX, and performed molecular and phenotypic characterization of this strain. In the M. loti MlccrM-OX infected plants, nodulation was apparently delayed at 7 days after inoculation (dai), however, the nodules that eventually formed on the MlccrM-OX roots showed nitrogen fixing ability by at least 21 dai. These results suggest that the initial morphogenic events were affected by ccrM-overexpression and that the correct pattern of DNA methylation of the bacterial genome is not essential for plant-microbe symbiosis, but are required for efficient nodulation.
Volume 389(2)
Pages 301-4
Published 2009-11-13
DOI 10.1016/j.bbrc.2009.08.137
PII S0006-291X(09)01734-3
PMID 19720053
MeSH DNA Methylation Epigenesis, Genetic* Genome, Bacterial Nitrogen Fixation / genetics* Rhizobium / genetics* Root Nodules, Plant / microbiology* Site-Specific DNA-Methyltransferase (Adenine-Specific) / genetics* Symbiosis
IF 2.705
Times Cited 4
Lotus / Glycine Miyakojima MG-20 M. loti