| RRC ID |
46876
|
| 著者 |
Imamura S, Hagiwara D, Suzuki F, Kurano N, Harayama S.
|
| タイトル |
Genetic transformation of Pseudochoricystis ellipsoidea, an aliphatic hydrocarbon-producing green alga.
|
| ジャーナル |
J Gen Appl Microbiol
|
| Abstract |
Pseudochoricystis ellipsoidea is a recently isolated unicellular green alga, which is classified within the family Trebouxiophyceae. This alga has a unique ability to synthesize and accumulate intracellularly a significant amount of aliphatic hydrocarbons. To elucidate molecular mechanisms of the hydrocarbon production in this organism, the development of genetic methods including DNA transformation methods are important. Towards the goal, we constructed several plasmids in which neomycin phosphotransferase II-encoding G418-resistant gene (nptII) is flanked by a P. ellipsoidea-derived promoter and terminator. These plasmids were introduced into P. ellipsoidea cells through particle-gun bombardment, and transformants were screened among G418-resistant cells by PCR amplification of plasmid-borne genes. Southern blot analysis demonstrated that the exogenous DNA was integrated into the genome of the transformants. Furthermore, the expression of nptII was confirmed at the transcript and protein levels by RT-PCR and immunoblot analyses, respectively. These results clearly indicated that a genetic transformation system was successfully established for P. ellipsoidea.
|
| 巻・号 |
58(1)
|
| ページ |
1-10
|
| 公開日 |
2012-1-1
|
| DOI |
10.2323/jgam.58.1
|
| PII |
JST.JSTAGE/jgam/58.1
|
| PMID |
22449745
|
| MeSH |
Blotting, Southern
Chlorophyta / drug effects
Chlorophyta / genetics*
Chlorophyta / growth & development
Chlorophyta / metabolism
Cloning, Molecular
DNA, Plant / genetics
DNA, Plant / metabolism
Gene Expression Regulation, Plant
Genes, Plant
Genetic Engineering / methods*
Genetic Markers
Genetic Vectors / genetics
Genetic Vectors / metabolism
Gentamicins / metabolism
Gentamicins / pharmacology
Hydrocarbons / metabolism*
Kanamycin Kinase / genetics
Kanamycin Kinase / metabolism
Plants, Genetically Modified / drug effects
Plants, Genetically Modified / genetics
Plants, Genetically Modified / growth & development
Plants, Genetically Modified / metabolism
Plasmids / genetics
Plasmids / metabolism
Promoter Regions, Genetic
Transformation, Genetic*
|
| IF |
1.442
|
| 引用数 |
12
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
|
