論文 - 詳細
| RRC ID | 46876 |
|---|---|
| 著者 | Imamura S, Hagiwara D, Suzuki F, Kurano N, Harayama S. |
| タイトル | Genetic transformation of Pseudochoricystis ellipsoidea, an aliphatic hydrocarbon-producing green alga. |
| ジャーナル | J Gen Appl Microbiol |
| Abstract |
Pseudochoricystis ellipsoidea is a recently isolated unicellular green alga, which is classified within the family Trebouxiophyceae. This alga has a unique ability to synthesize and accumulate intracellularly a significant amount of aliphatic hydrocarbons. To elucidate molecular mechanisms of the hydrocarbon production in this organism, the development of genetic methods including DNA transformation methods are important. Towards the goal, we constructed several plasmids in which neomycin phosphotransferase II-encoding G418-resistant gene (nptII) is flanked by a P. ellipsoidea-derived promoter and terminator. These plasmids were introduced into P. ellipsoidea cells through particle-gun bombardment, and transformants were screened among G418-resistant cells by PCR amplification of plasmid-borne genes. Southern blot analysis demonstrated that the exogenous DNA was integrated into the genome of the transformants. Furthermore, the expression of nptII was confirmed at the transcript and protein levels by RT-PCR and immunoblot analyses, respectively. These results clearly indicated that a genetic transformation system was successfully established for P. ellipsoidea. |
| 巻・号 | 58(1) |
| ページ | 1-10 |
| 公開日 | 2012-1-1 |
| DOI | 10.2323/jgam.58.1 |
| PII | JST.JSTAGE/jgam/58.1 |
| PMID | 22449745 |
| MeSH | Blotting, Southern Chlorophyta / drug effects Chlorophyta / genetics* Chlorophyta / growth & development Chlorophyta / metabolism Cloning, Molecular DNA, Plant / genetics DNA, Plant / metabolism Gene Expression Regulation, Plant Genes, Plant Genetic Engineering / methods* Genetic Markers Genetic Vectors / genetics Genetic Vectors / metabolism Gentamicins / metabolism Gentamicins / pharmacology Hydrocarbons / metabolism* Kanamycin Kinase / genetics Kanamycin Kinase / metabolism Plants, Genetically Modified / drug effects Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Plants, Genetically Modified / metabolism Plasmids / genetics Plasmids / metabolism Promoter Regions, Genetic Transformation, Genetic* |
| IF | 1.442 |
| 引用数 | 12 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | |