論文 - 詳細
| RRC ID | 469 |
|---|---|
| 著者 | Kômoto N, Sezutsu H, Yukuhiro K, Banno Y, Fujii H. |
| タイトル | Mutations of the silkworm molybdenum cofactor sulfurase gene, og, cause translucent larval skin. |
| ジャーナル | Insect Biochem Mol Biol |
| Abstract |
Normal silkworms (Bombyx mori) have opaque larval skin due to uric acid accumulation in the epidermis while a mutant, og, is translucent owing to a deficiency in xanthine dehydrogenase (XDH), which synthesizes uric acid. Molybdenum cofactor (MoCo) sulfurase is responsible for XDH activation in various organisms. A silkworm MoCo sulfurase gene was cloned and found to be on the og locus, whose mutant alleles, og(k) and og(t), show premature stop codons, proving that og is the MoCo sulfurase gene. It was observed that a miniature inverted-repeat transposable element (MITE), named Organdy, when inserted in an og(t) mutant allele exon, causes unstable splicing of a downstream intron leading to incomplete open reading frames. |
| 巻・号 | 33(4) |
| ページ | 417-27 |
| 公開日 | 2003-4-1 |
| DOI | 10.1016/s0965-1748(03)00006-7 |
| PII | S0965174803000067 |
| 解説 | MoCo sulfuraseはモリブデン補酵素硫黄化酵素をコードする。 o35系統(ogt/ogt)では、MoCo sulfuraseのエクソン４に551bpのトランスポゾンが挿入されている。o37系統（ogk/ogk)では、エクソン5の3'側とエクソン6の5'側を含む998 bpの領域が欠失している。 |
| PMID | 12650690 |
| MeSH | Amino Acid Sequence Animals Arabidopsis Proteins Base Sequence Bombyx / enzymology* Bombyx / genetics* Bombyx / growth & development Cloning, Molecular Conserved Sequence DNA Primers Genes Larva Molecular Sequence Data Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction / methods Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Skin Physiological Phenomena Sulfurtransferases / chemistry Sulfurtransferases / genetics* Sulfurtransferases / metabolism Xanthine Dehydrogenase / genetics |
| IF | 3.827 |
| 引用数 | 29 |
| WOS 分野 | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| カイコ | o35 o37 |