RRC ID 46931
Author Namiki Y, Ueno K, Mitani H, Virtudazo EV, Ohkusu M, Shimizu K, Kawamoto S, Chibana H, Yamaguchi M.
Title Scanning and negative-staining electron microscopy of protoplast regeneration of a wild-type and two chitin synthase mutants in the pathogenic yeast Candida glabrata.
Journal J Electron Microsc (Tokyo)
Abstract Protoplast regeneration of a wild-type and two mutant strains of Candida glabrata defective in CHS3 homologues encoding class IV chitin synthase in Saccharomyces cerevisiae was examined by scanning and negative-staining electron microscopy. In the wild-type strain, small particles and short filaments appeared on the protoplast surface at 10 min, filamentous materials covered the entire surface of the protoplast at 1 h, granular materials started filling interspaces of filamentous materials at 2 h and regeneration was completed at 6 h. The filamentous materials consisted of microfibrils of various widths ranging from ≤5 to 40 nm, and composed of β-glucan. Protoplasts of the two chitin synthase mutant strains of Δchs3A and Δchs3B completed regeneration essentially by the same process as wild-type strain, although it took more time. These results suggest that CHS3A and CHS3B genes may have important roles in cell wall formation during protoplast regeneration, but can be compensated by other cell wall enzymes.
Volume 60(2)
Pages 157-65
Published 2011
DOI 10.1093/jmicro/dfq082
PII dfq082
PMID 21216729
MeSH Candida glabrata / enzymology Candida glabrata / pathogenicity Candida glabrata / physiology Candida glabrata / ultrastructure* Cell Wall / metabolism* Cell Wall / ultrastructure Chitin Synthase / genetics* Chitin Synthase / metabolism Fungal Proteins / genetics Fungal Proteins / metabolism Glucans / ultrastructure Microfibrils / ultrastructure Microscopy, Electron Microscopy, Electron, Scanning Mutation* Negative Staining Protoplasts / physiology* Protoplasts / ultrastructure*
Times Cited 6
Pathogenic microorganisms NA