論文 - 詳細
| RRC ID | 46947 |
|---|---|
| 著者 | Yamaguchi M, Namiki Y, Okada H, Mori Y, Furukawa H, Wang J, Ohkusu M, Kawamoto S. |
| タイトル | Structome of Saccharomyces cerevisiae determined by freeze-substitution and serial ultrathin-sectioning electron microscopy. |
| ジャーナル | J Electron Microsc (Tokyo) |
| Abstract |
The cell structure has been studied using light and electron microscopies for centuries, and it is assumed that the whole structure is clarified by now. Little quantitative and three-dimensional analysis of cell structure, however, has been undertaken. We have coined a new word, 'structome', by combining 'structure' and '-ome', and defined it as the 'quantitative and three-dimensional structural information of a whole cell at the electron microscopic level'. In the present study, we performed structome analysis of Saccharomyces cerevisiae, one of the most widely researched biological materials, by using freeze-substitution and serial ultrathin-sectioning electron microscopy. Our analysis revealed that there were one to three mitochondria, ~220 000 ribosomes in a cell, and 13-28 endoplasmic reticula/Golgi apparatus which do not form networks in the cytoplasm in the G1 phase. Nucleus occupied ~10.5% of the cell volume; cell wall occupied ~17%; vacuole occupied ~5.8%; cytoplasm occupied ~64%; and mitochondria occupied only ~1.7% in the G1 phase. Structome analysis of cells would form a base for the post-genome research. |
| 巻・号 | 60(5) |
| ページ | 321-35 |
| 公開日 | 2011-1-1 |
| DOI | 10.1093/jmicro/dfr052 |
| PII | dfr052 |
| PMID | 21908548 |
| MeSH | Cell Nucleus / ultrastructure Cell Wall / ultrastructure Cellular Structures / ultrastructure Endoplasmic Reticulum / ultrastructure Freeze Substitution / methods* G1 Phase Golgi Apparatus / ultrastructure Imaging, Three-Dimensional Microscopy, Electron, Transmission / methods* Mitochondria / ultrastructure Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / ultrastructure* Vacuoles / ultrastructure |
| 引用数 | 31 |
| WOS 分野 | MICROSCOPY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | NA |