RRC ID 46947
Author Yamaguchi M, Namiki Y, Okada H, Mori Y, Furukawa H, Wang J, Ohkusu M, Kawamoto S.
Title Structome of Saccharomyces cerevisiae determined by freeze-substitution and serial ultrathin-sectioning electron microscopy.
Journal J Electron Microsc (Tokyo)
Abstract The cell structure has been studied using light and electron microscopies for centuries, and it is assumed that the whole structure is clarified by now. Little quantitative and three-dimensional analysis of cell structure, however, has been undertaken. We have coined a new word, 'structome', by combining 'structure' and '-ome', and defined it as the 'quantitative and three-dimensional structural information of a whole cell at the electron microscopic level'. In the present study, we performed structome analysis of Saccharomyces cerevisiae, one of the most widely researched biological materials, by using freeze-substitution and serial ultrathin-sectioning electron microscopy. Our analysis revealed that there were one to three mitochondria, ~220 000 ribosomes in a cell, and 13-28 endoplasmic reticula/Golgi apparatus which do not form networks in the cytoplasm in the G1 phase. Nucleus occupied ~10.5% of the cell volume; cell wall occupied ~17%; vacuole occupied ~5.8%; cytoplasm occupied ~64%; and mitochondria occupied only ~1.7% in the G1 phase. Structome analysis of cells would form a base for the post-genome research.
Volume 60(5)
Pages 321-35
Published 2011
DOI 10.1093/jmicro/dfr052
PII dfr052
PMID 21908548
MeSH Cell Nucleus / ultrastructure Cell Wall / ultrastructure Cellular Structures / ultrastructure Endoplasmic Reticulum / ultrastructure Freeze Substitution / methods* G1 Phase Golgi Apparatus / ultrastructure Imaging, Three-Dimensional Microscopy, Electron, Transmission / methods* Mitochondria / ultrastructure Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / ultrastructure* Vacuoles / ultrastructure
Times Cited 11
WOS Category MICROSCOPY
Resource
Pathogenic microorganisms NA